ZuoTi.Pro
Question

For each of the following, what are you running on the gel, and what is being used as the probe? (DNA, RNA, Protein, or Antib

science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

1.SOUTHERN BLOTTING.

it is a technique which is use to either identify the DNA of interest from a mixture of DNA samples or to identify a specific base sequence from a particular strand of DNA. hence, we run DNA fragments in the gel and use labeled single DNA fragment as probe having sequence complementary to the target DNA.

2. WESTERN BLOTTING.

it is a technique use to detect or identify a specific protein in the given sample. hence, we run protein in the gel and use labeled antibody as probe which is specific to theprotein of interest.

3. NORTHERN BLOTTING.

it is a technique use to detect a particular RNA from the given sample. hence, we run RNA in the gel and use labeled RNA or ssDNA fragments as probe which have complimentary sequences to the target RNA.

0 0
Add a comment
Know the answer?
Add Answer to:
For each of the following, what are you running on the gel, and what is being...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • 1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2....

    1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2. Which vehicles are often used for gene therapy to carry a healthy gene? Bacteria Viruses Plastic capsules Both A and B Both A and C 3. A common method in biochemistry to detect and analyze proteins is: Northern blot Southern blot Western blot Eastern blot All of the above 4. The technique used to identify a specific DNA sequence in bacterial colonies is In...

  • Please explain what might causes the following results A. You have bright band on a Northern...

    Please explain what might causes the following results A. You have bright band on a Northern Blot for a specific RNA, but the Wester blot Shows no band for the protein encoded by that RNA. B. Your Western blot show that your protein increases size in extracts from cancer cell vs. normal cells. C. Your Northern blow shows different sized fragments in tissue extracted from brain cells vs heart muscles.

  • Which of the following statements is FALSE about Southern blotting? a) smaller nucleic acid fragments move...

    Which of the following statements is FALSE about Southern blotting? a) smaller nucleic acid fragments move faster than larger nucleic acid fragments in gels b) a hybridization probe for Southern blot can be single stranded DNA or RNA c) In most cases, DNA is cut with a restriction enzyme prior to gel electrophoresis d) smaller hybridization probes will always detect smaller DNA fragments than larger probes e) Southern blotting is used to analyze DNA

  • Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting,...

    Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...

  • If you wanted to find a protein that binds RNA to perhaps regulate RNA degradation or...

    If you wanted to find a protein that binds RNA to perhaps regulate RNA degradation or translation, you could test protein binding this RNA by __. (application question) A Gel shift B Southern C Northern D Western If one performed co-immunoprecipitation where antibodies were used to precipitate 1) TFIID (20 kD), 2) TFII B (40 kD) or 3) TFII H (50 kD) and then a western was performed with the TFII B antibody, which lanes (1,2, 3 or combination below)...

  • Which technique would you use if you wanted to < Identify all the regions of open...

    Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...

  • 9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can...

    9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can be detected by a. ELISA b. flow cytometry c. RFLP d. FISH 10. Which of the following techniques enables the identification of a particular sequence of nucleic acid on a cell or tissue? a. northern blot b. western Blot c. FISH d. southern blot 11. The polymerase chain reaction is used to a. convert RNA back into DNA b. amplify a target nucleic acid...

  • QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...

    QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...

  • a. What organisms were the tubulin and the cyclin primary antibodies raised in? b. What was the purpose of the tubulin c...

    a. What organisms were the tubulin and the cyclin primary antibodies raised in? b. What was the purpose of the tubulin control? What other proteins might you have used instead of tubulin? The aim of this week’s experiment is to verify how M-cyclin is varying within the synchronised tobacco BY2 cell culture. Protein levels will be assays using a dot blot immunoassay in which a crude protein extract is coated onto a nitrocellulose membrane and probed with specific antibodies. This...

  • B. Based on lanes 4 and 3, which cell type has the more tubulin protein-NIH/3T3 cells?...

    B. Based on lanes 4 and 3, which cell type has the more tubulin protein-NIH/3T3 cells? Co cells? About the same? Can you tell? 14. As a way of contrasting the results of various molecular methods, answer each of the following questions. Note that each of these questions asks you to think about two methods that might seem superficially to yield similar information, but to focus how the results are different. A) What information would you get from a northern...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT