3 usage of recombination DNA except insulin
Holiday junction (HJ) is a very important mechanism for natural DNA recombination. HJ catalyzes DNA recombination between _________ using a special protein complex. Select one: a. two dsDNA b. one dsDNA and one ssDNA c. two ssDNA d. All of these e. None of these
Compare and contrast the 3 bacterial gene transfers with DNA recombination. Explain why these are not reproduction, and why they are important to bacterial species.
How does homologous recombination of new bacteria DNA occur?
Which of the following is NOT a function of homologous recombination? Repair of DNA double strand breaks Repairing bulky DNA damage Pairing of homologous chromosomes in meiosis Rescue of collapsed replication forks
The process of putting a recombinant DNA molecule into a cell is called... Select one: Recombination Transformation Ligation Insertional Inactivation In a Southern blot... Select one: Protein is transferred from a gel to a nylon and hybridized to a DNA probe DNA is transferred from a gel to a nylon and hybridized to a DNA probe RNA is transferred from a gel to a nylon and hybridized to a DNA probe One strand of double-stranded DNA is shown below. Assume...
8. DNA cloning. The first DNA clone using recombinant DNA technology was human insulin, which was made in 1978. The insulin gene was inserted into a plasmid and the gene was transcribed and translated from the plasmid in E coll cells. Insulin is synthesized by ribosomes in human pancreatic cells. Insulin is synthesized as a longer polypeptide, which is then trimmed by proteases to make the mature chains A and B (see Fig. 2.17 on page 39 of your textbook)....
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______ a) meiosis b) mitosis c) both 3. Homologous recombination in meiosis has the main purpose of_____ a) DNA repair b) Creating new chromosomes c) Sealing double-stranded breaks 4. Strand invasion usually happens without enzymatic assistance. T/F? 5. When replication fork runs into a nick, it results in a_______ a) single-stranded break b) double-stranded break 6. The invading end is usually a _______ a) 3'...
Explain the general process of homologous recombination and understand that this process only occurs with DNA-based viruses.
Describe 2 meiotic sources of recombination? Why is plasmid based engineered DNA considered recombinant?
Sort the following steps for repairing double-strand breaks by homologous recombination a) Ligation b) DNA synthesis using undamaged DNA as template c) DNA synthesis using original DNA as template d) Release of the invading strand e) Strand invasion f) Nuclease digestion (resection)