Sort the following steps for repairing double-strand breaks by homologous recombination a) Ligation b) DNA synthesis...
Sort the following steps for repairing double-strand breaks by homologous recombination
a) Ligation
b) DNA synthesis using undamaged DNA as template
c) DNA synthesis using original DNA as template
d) Release of the invading strand
e) Strand invasion
f) Nuclease digestion (resection)
Homework Answers
Steps for repairing double-strand breaks by homologous recombination:
f) Nuclease digestion (resection)
e) Strand invasion
b) DNA synthesis using undamaged DNA as template
d) Release of the invading strand
c) DNA synthesis using original DNA as template
a) Ligation
Add Answer to:
Sort the following steps for repairing double-strand breaks by
homologous recombination
a) Ligation
b) DNA synthesis...
In cells that are exposed to sunlight, ultraviolet (UV) light can result in covalent linkage between...
In cells that are exposed to sunlight, ultraviolet (UV) light can result in covalent linkage between two adjacent DNA bases. If not repaired in time, such dimers can lead to inheritable mutations. Consider the sequence 5'-GGTATGATCATTATAA-3' in the chromosome of a cell that is exposed to intense sunlight. How many possible dimers can form in the region of DNA double helix corresponding to this sequence? 04 O3 Sort the following steps in the order that they normally happen during the...
Which of the following is NOT a function of homologous recombination? Repair of DNA double strand...
Which of the following is NOT a function of homologous recombination? Repair of DNA double strand breaks Repairing bulky DNA damage Pairing of homologous chromosomes in meiosis Rescue of collapsed replication forks
The process of homologous recombination is a means to repair double-strand breaks, the most lethal form...
The process of homologous recombination is a means to repair double-strand breaks, the most lethal form of DNA damage. Arrange the sequence of events in the proper order: 1. ATR recognizes single-stranded ends, binds to them, and initiates signaling. 2. RAD51 complexes with ssDNA and searches for homologous sequences. 3. MRN nuclease trims the 5' ends, leaving ss-3' ends that are coated with replication protein A. 4. ATM activates a nuclease in MRN and signals downstream effectors. 5. RAD51-ssDNA complex...
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______...
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______ a) meiosis b) mitosis c) both 3. Homologous recombination in meiosis has the main purpose of_____ a) DNA repair b) Creating new chromosomes c) Sealing double-stranded breaks 4. Strand invasion usually happens without enzymatic assistance. T/F? 5. When replication fork runs into a nick, it results in a_______ a) single-stranded break b) double-stranded break 6. The invading end is usually a _______ a) 3'...
Haploid yeast cells that preferentially repair double-strand breaks by homologous recombination (rather than by non-homologous end...
Haploid yeast cells that preferentially repair double-strand breaks by homologous recombination (rather than by non-homologous end joining) are especially sensitive to agents that cause double-strand breaks in DNA. If the breaks occur in the G1 phase of the cell cycle, most cells will die; however, if the breaks occur in the G2 phase, a much higher fraction of cells survive. Why do you suppose this is?
z Instructions Question 1 (Q039) In addition to the repair of DNA double-strand breaks, homologous recombination...
z Instructions Question 1 (Q039) In addition to the repair of DNA double-strand breaks, homologous recombination is a mechanism fe generating genetic diversity by swapping segments of parental chromosomes. During which process does swapping occur? DNA replication ODNA repair O meiosis transposition No new data to save. Last checked at 8:05am
Ionizing radiation and oxidative damage can cause DNA double-strand breaks. A. True. B False Nucleotide Excision...
Ionizing radiation and oxidative damage can cause DNA double-strand breaks. A. True. B False Nucleotide Excision Repair involves recognition of distortions in the DNA double helix rather than specific base changes. A. True. B False Homologous recombination does not occur in prokaryotic cells. A. True. B False Many chemotherapeutic agents are paradoxically DNA damaging agents A. True. B False Meiot.c recombination starts with, double-strand break caused by errors In DNA replication A. True. B False ONA replication origins are typically...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10 The form of genetic reco...
QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10 The form of genetic recombination that allows movement of genetic elements from one DNA site to another is termed: O A site-specific recombination O B. homologous recombination ° C. branch migration D.transposition
QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10...
Genetics: include pictures please.(: For each of the following steps of recombination at the molecular level,...
Genetics: include pictures please.(:
For each of the following steps of recombination at the molecular level, briefly describe what is happening and draw out what the chromatids look like (just the DNA, don't need to draw out the proteins), and what proteins are involved in each step. 1. Double strand break formation 2. Resection 3. First strand invasion 4. Formation of the double Holliday junction 5. Branch migration 6. Resolution
In cells that are exposed to sunlight, ultraviolet (UV) light can result in covalent linkage between two adjacent DNA bases. If not repaired in time, such dimers can lead to inheritable mutations. Consider the sequence 5'-GGTATGATCATTATAA-3' in the chromosome of a cell that is exposed to intense sunlight. How many possible dimers can form in the region of DNA double helix corresponding to this sequence? 04 O3 Sort the following steps in the order that they normally happen during the...
The process of homologous recombination is a means to repair double-strand breaks, the most lethal form of DNA damage. Arrange the sequence of events in the proper order: 1. ATR recognizes single-stranded ends, binds to them, and initiates signaling. 2. RAD51 complexes with ssDNA and searches for homologous sequences. 3. MRN nuclease trims the 5' ends, leaving ss-3' ends that are coated with replication protein A. 4. ATM activates a nuclease in MRN and signals downstream effectors. 5. RAD51-ssDNA complex...
Haploid yeast cells that preferentially repair double-strand breaks by homologous recombination (rather than by non-homologous end joining) are especially sensitive to agents that cause double-strand breaks in DNA. If the breaks occur in the G1 phase of the cell cycle, most cells will die; however, if the breaks occur in the G2 phase, a much higher fraction of cells survive. Why do you suppose this is?
z Instructions Question 1 (Q039) In addition to the repair of DNA double-strand breaks, homologous recombination is a mechanism fe generating genetic diversity by swapping segments of parental chromosomes. During which process does swapping occur? DNA replication ODNA repair O meiosis transposition No new data to save. Last checked at 8:05am
Ionizing radiation and oxidative damage can cause DNA double-strand breaks. A. True. B False Nucleotide Excision Repair involves recognition of distortions in the DNA double helix rather than specific base changes. A. True. B False Homologous recombination does not occur in prokaryotic cells. A. True. B False Many chemotherapeutic agents are paradoxically DNA damaging agents A. True. B False Meiot.c recombination starts with, double-strand break caused by errors In DNA replication A. True. B False ONA replication origins are typically...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10 The form of genetic recombination that allows movement of genetic elements from one DNA site to another is termed: O A site-specific recombination O B. homologous recombination ° C. branch migration D.transposition
QUESTION 9 Strand invasion requires a__, O A. 5', single-stranded B. 5', double-stranded ° C. 3, single-stranded O D.3', double-stranded DNA molecule. QUESTION 10...
Genetics: include pictures please.(:
For each of the following steps of recombination at the molecular level, briefly describe what is happening and draw out what the chromatids look like (just the DNA, don't need to draw out the proteins), and what proteins are involved in each step. 1. Double strand break formation 2. Resection 3. First strand invasion 4. Formation of the double Holliday junction 5. Branch migration 6. Resolution
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