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A suspension of yeast cells is being grown under anaerobic conditions such that glucose is degraded...

A suspension of yeast cells is being grown under anaerobic conditions such that glucose is degraded to ethanol and carbon dioxide.
Adding an inhibitor of alcohol dehydrogenase to the yeast cell suspension results in rapid cell death. Why?

a.) Without alcohol dehydrogenase, the acetaldehyde concentration increases to levels that are toxic to the yeast cells.

b.) NADH generated during glycolysis cannot be recycled back to NAD+, thus anaerobic ATP production through glycolysis cannot be maintained.

c.) Pyruvate is no longer being produced for use in gluconeogenesis, so the cells quickly deplete this energy source.

d.) Loss of this enzyme during anaerobic glucose degradation leads to an increased requirement for ATP, which cannot be met.

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Answer #1

b.) NADH generated during glycolysis cannot be recycled back to NAD+, thus anaerobic ATP production through glycolysis cannot be maintained.

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