Shine-Dalgarno (SD) has a role in translation by binding to the 16S ribosomal RNA. What would...
Shine-Dalgarno (SD) has a role in translation by binding to the 16S ribosomal RNA. What would happen if half of the SD was removed?
Homework Answers
Shine Dalgarno sequence is located 10bp upstream from the translation initiation codon in the bacterial mRNA. It is the sequence where the 3' end of the ribosome subunit -30S basepairs with mRNA inorder to initiate the translation and position the AUG codon to the P site of the ribosome.
So if the Shine Dalgarno sequence is removed partially, translation - gene expression of proteins will reduce or completely halt. Translation will not take place.
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Shine-Dalgarno (SD) has a role in translation by binding to the
16S ribosomal RNA. What would...
102. Fill in the chart comparing translation in prokaryotes vs. eukaryotes. Prokaryotes Eukaryotes Small ribosomal subunit...
102. Fill in the chart comparing translation in prokaryotes vs. eukaryotes. Prokaryotes Eukaryotes Small ribosomal subunit Large ribosomal subunit Energy source Shine Dalgarno sequence Kozak sequence 5' cap binding protein Poly-A tail binding protein Protein factors that bind ribosome Start codon Initiator tRNA Elongation factor proteins eEF -1 eEF 2 EF- Tu EF G Termination codons eRF RF- 1 RF 2 RF 3
In the PCR exercise you described why we amplify the 16s ribosomal RNA gene. What other...
In the PCR exercise you described why we amplify the 16s ribosomal RNA gene. What other genes might help us to identify specific bacteria? In other words, what traits could be sequenced that are unique to bacteria?
Match the following choices to the questions below. UUU catalyzes translocation of the ribosome involved in...
Match the following choices to the questions below. UUU catalyzes translocation of the ribosome involved in transcription associated with the binding of mRNA in the ribosome binds to Shine-Dalgarno sequence involved in replication contains information in the form of anticodons catalyzes the formation of amino acid-AMP AUG UAG binds to pribnow box catalyzes formation of peptide bonds catalyzes disassembly of the translation complex associated with the binding of tRNA in the ribosome catalyzes disassembly of the transcription complex start codon...
In Bacteria, translation of nascent RNA transcripts is often initiated before transcription is terminated. When this...
In Bacteria, translation of nascent RNA transcripts is often initiated before transcription is terminated. When this occurs: Select one: a. RNA is transcribed 3'=>5' and amino acids are added to the carboxy terminus of the growing polypeptide as the ribosome moves 5' => 3' along the mRNA b. RNA is transcribed 5'->3' and amino acids are added to the amino terminus of the growing polypeptide as the ribosome moves 3'=> 5' along the mRNA c. RNA is transcribed 3'=> 5'...
1.What sequence upstream of AUG in prokaryotic mRNA facilitates recruitment of the 30S ribosomal subunit by...
1.What sequence upstream of AUG in prokaryotic mRNA facilitates recruitment of the 30S ribosomal subunit by base pairing with 16S ribosomal RNA? A. The Pribnow box B. The Shine Dalgarno sequence C. The TATA box D. The -35 region E. A stop codon 2.What is the role of the Shine-Delgarno sequence in prokaryotic mRNAs? It specifies the site of translation initiation - ? It specifies the site of polyA addition RNA splicing RNA export It specifies the site of translation...
QUESTION 35 Tetracycline is an antibiotic used to treat bacterial infections in humans because it prevents...
QUESTION 35 Tetracycline is an antibiotic used to treat bacterial infections in humans because it prevents the growth and spread of bacteria. This drug is known to bind strongly and specifically to the A site in the 30S ribosomal subunit. Based on this information you would expect that: a. tetracycline does not affect translation in eukaryotic cells. b. tetracycline inhibits RNA splicing in prokarytes. c. tetracycline will prevent the small ribosomal subunit from binding to the Shine-Dalgarno sequence. d. tetracycline...
QUESTION 1 QUESTION 5 QUESTION 11 Identify the components required for translation initiation in bacteria What...
QUESTION 1 QUESTION 5 QUESTION 11 Identify the components required for translation initiation in bacteria What is the enzymatic component of the ribosome? A Protein Identify the TRANS components of the transcription initiation complex in bacteria ATFIE Bir RNA C. TATA BOX D-10 and 35 sequences E Signa factor B. Carbohydrates C.RNA CATFIE B. 5methyl guanosine cap C. Shine-Dalgamo Sequence D. Sigma factor CETFIID (TBP and TAFS) FTFIIB G. Initiator RNA H.10 and 35 sequences EL Smal ribosomal subunit J....
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7)...
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7) During translation elongation in procaryotes, the interplay between EF-Tu and EF-TS regulate tRNA charging to the A site of the ribosome. Please describe in detail the sequence of events that take place during tRNAEFTU GTR ternary complex formation. Explain how the EF-Tu GDP that is discharged from the ribosome is converted to EF- Tu GTR that is the competent complex for translation elongation? What is...
3. Briefly describe the importance of accurate splicing. What happens if too much RNA is removed during splicing? What happens if not enough RNA is removed during splicing? What would happen if a...
3. Briefly describe the importance of accurate splicing. What happens if too much RNA is removed during splicing? What happens if not enough RNA is removed during splicing? What would happen if a splicing event results in the deletion of a single nucleotide from the beginning of an exon? (4 points)
3. Briefly describe the importance of accurate splicing. What happens if too much RNA is removed during splicing? What happens if not enough RNA is removed during splicing? What...
1. Describe the differences between prokaryotic and eukaryotic translation initiation. How does the ribosome find the...
1. Describe the differences between prokaryotic and eukaryotic translation initiation. How does the ribosome find the correct start codon and what proteins are involved in the process? please include the shine-dalgarno sequence in the answer. 2. Consider the following partial sequence of messenger RNA. The sequence below contains the code for a short, complete protein. 5 ́-UCCCCAGUCAUGGAGUCGUUAAUUAAAUGACCGGUGCGGAUCGUA - 3 ́ Using the codon chart (from your textbook or in the lecture slides), give the amino acid sequence of the protein...
102. Fill in the chart comparing translation in prokaryotes vs. eukaryotes. Prokaryotes Eukaryotes Small ribosomal subunit Large ribosomal subunit Energy source Shine Dalgarno sequence Kozak sequence 5' cap binding protein Poly-A tail binding protein Protein factors that bind ribosome Start codon Initiator tRNA Elongation factor proteins eEF -1 eEF 2 EF- Tu EF G Termination codons eRF RF- 1 RF 2 RF 3
Match the following choices to the questions below. UUU catalyzes translocation of the ribosome involved in transcription associated with the binding of mRNA in the ribosome binds to Shine-Dalgarno sequence involved in replication contains information in the form of anticodons catalyzes the formation of amino acid-AMP AUG UAG binds to pribnow box catalyzes formation of peptide bonds catalyzes disassembly of the translation complex associated with the binding of tRNA in the ribosome catalyzes disassembly of the transcription complex start codon...
In Bacteria, translation of nascent RNA transcripts is often initiated before transcription is terminated. When this occurs: Select one: a. RNA is transcribed 3'=>5' and amino acids are added to the carboxy terminus of the growing polypeptide as the ribosome moves 5' => 3' along the mRNA b. RNA is transcribed 5'->3' and amino acids are added to the amino terminus of the growing polypeptide as the ribosome moves 3'=> 5' along the mRNA c. RNA is transcribed 3'=> 5'...
QUESTION 35 Tetracycline is an antibiotic used to treat bacterial infections in humans because it prevents the growth and spread of bacteria. This drug is known to bind strongly and specifically to the A site in the 30S ribosomal subunit. Based on this information you would expect that: a. tetracycline does not affect translation in eukaryotic cells. b. tetracycline inhibits RNA splicing in prokarytes. c. tetracycline will prevent the small ribosomal subunit from binding to the Shine-Dalgarno sequence. d. tetracycline...
QUESTION 1 QUESTION 5 QUESTION 11 Identify the components required for translation initiation in bacteria What is the enzymatic component of the ribosome? A Protein Identify the TRANS components of the transcription initiation complex in bacteria ATFIE Bir RNA C. TATA BOX D-10 and 35 sequences E Signa factor B. Carbohydrates C.RNA CATFIE B. 5methyl guanosine cap C. Shine-Dalgamo Sequence D. Sigma factor CETFIID (TBP and TAFS) FTFIIB G. Initiator RNA H.10 and 35 sequences EL Smal ribosomal subunit J....
6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7) During translation elongation in procaryotes, the interplay between EF-Tu and EF-TS regulate tRNA charging to the A site of the ribosome. Please describe in detail the sequence of events that take place during tRNAEFTU GTR ternary complex formation. Explain how the EF-Tu GDP that is discharged from the ribosome is converted to EF- Tu GTR that is the competent complex for translation elongation? What is...
3. Briefly describe the importance of accurate splicing. What happens if too much RNA is removed during splicing? What happens if not enough RNA is removed during splicing? What would happen if a splicing event results in the deletion of a single nucleotide from the beginning of an exon? (4 points)
3. Briefly describe the importance of accurate splicing. What happens if too much RNA is removed during splicing? What happens if not enough RNA is removed during splicing? What...
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