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you are working for a major chemical company and you are asked to perform an Ames...

you are working for a major chemical company and you are asked to perform an Ames test for a newly developed cleaner. if you were to use his His auxotrophic mutants for your Ames test, you would need to include a minimal amount of histidine in your plates. why is this necessary?
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Answer #1

Auxotrophic mutant is a his-organism they cant produce histidine themselves so the supplement of histidine in the plate is inevitable. Histidine helps the growth of the organism and helps to the cell division as well, which is essential for the mutation to occur.

thank you for the opportunity

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