What other features of pGap besides the GAPC gene can you infer have been bioengineered in...
What other features of pGap besides the GAPC gene can you infer have been bioengineered in the plasmid? Explain your reasoning and be sure to provide a list of every possible feature that you can infer has been added to this plasmid.
Homework Answers
Solution:-
Other features of pGAP besides the GAPC gene that have been bioengineered in the plasmid are:
It contains CamR gene, ColE1 gene, CYC1 terminator sequence and TEF1 promoter sequence. CamR gene is the chloramphenicol resistance gene and provides resistance to the antibiotic chloramphenicol. CYC1 terminator sequence is a high capacity terminator sequence obtained from yeast which improves expression of genes. TEF1 promoter sequence from yeast shows high degree of gene expression.
pJET contains AmpR gene which provides resistance to the antibiotic ampicillin and also the lac promoter sequence which shows high level of gene expression.
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