A test tube with a suspension of cells that was diluted to 1:100 (DF 1/100). You...
A test tube with a suspension of cells that was diluted to 1:100 (DF 1/100). You performed a cell count using a hemocytometer chamber. Assuming that the count was 15,14,18,20,22 cells in 5 of 1/25 boxes. What is the suspension concentration?
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A test tube with a suspension of cells that was diluted to 1:100
(DF 1/100). You...
You have been given a yeast suspension to count. You count the number of yeast cells...
You have been given a yeast suspension to count. You count the number of yeast cells in 7 of the 0.04mm2 boxes of the central grid of the hemocytometer. Your counting results are: 20, 18, 19, 21, 22, 17, and 22. Assuming there has been no dilution and you are counting the original sample, determine the concentration of cells in the original sample Prepare a set of serial dilutions of the yeast suspension for a viable count. Write and draw...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an...
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in the 16 little squares. Please explain how to get each! a. Calculate the concentration of the cells prior to the dilution. Show your calculations. Use scientific notation to express the result. b. Now, you want to seed a 25cm2 flask with 750,000 cells. Calculate how many ml...
A total volume of 1 mL of cell suspension was used for this exercise. Blood was...
A total volume of 1 mL of cell suspension was used for this exercise. Blood was diluted 1/10. The cells were then serially diluted using saline. -How was 1/10 cell suspension made? -How was 1/100 cell suspension made? -What is the difference between a 1/100 and 1/1000 cell suspension?
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
this is a bacterial growth curve experiment . please explain rhe results Procedure: You will follow...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube...
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. 1b Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
You notice that Alice wanted to check the cell number after one hour of growth, so...
You notice that Alice wanted to check the cell number after one hour of growth, so she also used a Petroff-Hauser chamber (Hemocytometer) to count the number of cells. If she had 10^4 cells in the original culture tube after one hour, how many microliters (uL) of the original undiluted culture would she need to be able to count approximately 100 cells?
Your sample will now be diluted to 1:2000 with an isotonic saline solution. Furthermore, when using...
Your sample will now be diluted to 1:2000 with an isotonic
saline solution. Furthermore, when using a hemocytometer to count
RBCs, only five squares of the central field are counted as
indicated by the following diagram
(Total # of cells counted)(10/# of 1 mm^2 fields
counted)(dilution factor) = # of cells/ul
by the following diagram: 400X 100X Figure 4: Pattern used for counting RBCs in the center field of a hemocytometer. Upon loading your diluted sample into the hemocytometer, these...
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of...
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. Pipetting less than 1 μL is hard to do accurately. Do a dilution if less than 1μL After dilution,Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
Your sample will now be diluted to 1:2000 with an isotonic
saline solution. Furthermore, when using a hemocytometer to count
RBCs, only five squares of the central field are counted as
indicated by the following diagram
(Total # of cells counted)(10/# of 1 mm^2 fields
counted)(dilution factor) = # of cells/ul
by the following diagram: 400X 100X Figure 4: Pattern used for counting RBCs in the center field of a hemocytometer. Upon loading your diluted sample into the hemocytometer, these...
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