The following data on bakers’ yeast in a particular medium at 23.4 °C and various oxygen partial pressures were obtained:
Po2 | Qo2 (no sulfanilamide) | Qo2, (20 mg sulfanilatnide/mL added to medium) |
0.0 | 0.0 | 0.0 |
0.5 | 23.5 | 17.4 |
1.0 | 33.0 | 25.6 |
1.5 | 37.5 | 30.8 |
2.5 | 42.0 | 36.4 |
3.5 | 43.0 | 39.6 |
5.0 | 43.0 | 40.0 |
P02 oxygen partial pressure, mmHg; Q02 = oxygen uptake rate, µL of O2 per hour per mg of cells.
(a) Calculate the Q0 maximum (Vmax), and the Michaelis-Menten constant Km. (Ahs.: V„,ax = 52.63 µL O2/hvmg cells.)
(b) Using the Lineweaver-Burk plot, determine the type of inhibition sulfanilamide that causes the O2 uptake to change.
(c) List ways you can work this problem incorrectly.
(d) How could you make this problem more difficult?
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