The transcription start sites and polyadenylation sites are indicated in the below figure:
The gel analysis of the Ribonucleic acid (RNA) isolated from the cells gives the following results:
a. The Deoxyribonucleic acid (DNA) element that permits activation by Fibroblast Growth Factor (FGF) is seen in Clone A, but not in B, C and D. This specifies that the activation site for DNA binding is located between the end of exon 1 [E1(f)] and the end of exon 3 [E3(f)].
b. The Deoxyribonucleic acid (DNA) element that permits repression by cortisol of Clone A and B but not C. As Clone D is intact globin gene, the effect on D is not absorbed. By analyzing the above results, it conforms that the site of DNA repression is located in between region of E3(f) or the flanking sequences of this gene.
c. The Deoxyribonucleic acid (DNA) element that permits induction by erythropoietin (EP) is seen in Clones C and D but no in B. As clone A is intact with c-fos gene in response to EP, the effect on A is not absorbed. This specifies that the induction site for DNA binding is localized near side of E3(g) or the flanking regions of the globin gene.