Problem

Solutions For An Introduction to Genetic Analysis Chapter 8 Problem 16P

Step-by-Step Solution

Solution 1

a) The linear plasmid showing two strands running anti-parallel to each other from to direction in opposite directions.

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b) The genes would be transcribed from the - direction and the template strand would be running from the - direction. The figure shows the template strand that would be used in the synthesis of mRNA transcripts.

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Step #3 of 4

c) The transcription initiation site is the one where the ribonucleic acid (RNA) polymerase enzyme fixes itself to the deoxyribonucleic acid (DNA) and initiates the process of transcription. It is at the -35 and -10 region in the DNA molecule the RNA polymerase enzyme would be fixed through the involvement of sigma factor.

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Step #4 of 4

d) The growing mRNA strand would be elongated till it reaches the end of the gene. The mRNA molecule would be growing from the - direction with the template running in an anti-parallel way to the growing mRNA molecule.