Question

The sequence below represents the first section of the template strand of DNA of a structural gene in an prokaryotic organism. Position +1 is shown in orange. Please fill in the blanks that correspond YOUR RESPONSES SHOULD ALL BE IN UPPER CASE. Amino acid sequences should be written in the format ALA-TYR-LEU Stop codon is not written 3 GTAACTĀTAATTACGCGTATAATGAT 5 What would be the nucleotides that form the promoter? What would be the 5'UTR sequence? What would be the sequence of amino acids coded by this section of the gene?

Answer 1

TATAAT are the nucleotides Promoter. DNA template :- 3' GTAACTATAATTACGCG TATAATGAT S' MRNA sequence, that is transeribed from the above DNA templat sequence will be i mRNA sequence i 5'CA LUGAUA LUAA UGCGCAUA QUACUA 3 start codon 5 UTR sequence 5 untranslated region is directly upstream from the initiation codon Cie, AUG)

3 Prokaryotes contain three Promotor elements: one upstream to the gene, second To nucleotides downstream to it, and the third 35 nucleotides downstream to it - The s untranslated region (S'UTR) is the region of an MRNĚ that is directly upstream from the initiation codon. Initiation codon is the triplet of nucleotide that helps in initiation of translation process. In DNA molecule, upstream is toward. the 5' end of the coding strand for the gene and downstream is toward the 3 end. > Triplet of nucleotide in the mRNA sequence codes for different amino aud. This triplet of nucleotide called as codon.
Translation process that is protein synthesis initiated by start Codon (i.e, AUG) amino acids formed will be Methionine Arginine Isoleucine Leucine Leucine

AUG codes for Methionine

CGC codes for Arginine

AUA codes for Isoleucine

UUA codes for Leucine

CUA codes for Leucine