Explain the mechanism of how substrate concentration affects enzyme activity and how this mechanism yields the expected summary curve
Explain the mechanism of how substrate concentration affects enzyme activity and how this mechanism yields the...
Review your knowledge of how enzyme activity is affected by substrate concentration by selecting the correct statements concerning enzyme activity at low substrate concentrations. Contact between enzyme and substrate is rare Velocity of the reaction increases Enzyme makes product rapidly Enzyme makes product slowly Enzyme is saturated with substrate
Annabeth is testing the effect of substrate concentration on enzyme activity using cellobiase and p-nitrophenyl glucopyranoside. After she adds STOP solution to her reactions and measures the absorbances, she discovers that one sample has an absorbance that is beyond the range of her standard curve. How can Annabeth determine the concentration of product for this sample?
Can you infinitely infrease the substrate concentration to increase enzyme activity in a closed system such as a batch culture? Can you infinitely increase the substrate concentration to increase enzyme activity in a closed system such as a batch culture? Yes Your answer B NO
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
COST-LAB QUESTIONS: 1. What happens to enzyme activity as the enzyme concentration is increased? 2. Based on your results, what is the optimum pH for lactase? 3. Do all enzymes in the body have the same optimum pH? Why or why not? 4. What happens to enzyme activity as the substrate concentration is increased? Explain why this occurs.
1 Consider this graph that we talked discussed in class on how an enzymes affects the rate of product formation. Catalyzed Uncatalyzed reaction Substrate concentration Now consider you have discovered an allosteric inhibitor of the enzyme. You do this experiment again, adding enough allosteric inhibitor to block 50% of the enzyme activity present in the reaction. a. Thinking in terms of the models shown in class, what does it actually mean in molecular terms when "50% of the enzymatic activity"...
explain the induced-fit model of enzyme action, and how this differe from the "Lock-&-key" model NOA HOJAVEN EN GENOSSO Questions to expand your mind. 1. Explain the Induced-Fit model of enzyme action, and how this differs from the Lock-&-Key" model. 2. Distinguish between catabolic and anabolie enzyme reactions 3. Define and describe 3 variables that may affect enzyme activity. 4. **Online Search Opportunity** What are the steps involved in commercial cheese making? Is this different from how organic farm cheese...
List and describe the factors that can affect enzymatic reactions. a. Substrate Concentration b. Enzyme Concentration c. pH d. Temperature e. Cofactors f. Inhibitors (I have them listed but do not understand their affects)
Enzyme activity will be high when substrate concentration is equal to KM b. below KM c. above KM d. none of the above Metalloproteases mostly use the element Cu b. Fe c. Zn d. Mn
3. Why is an allosteric enzyme more sensitive to substrate concentration around Km values than a Michaelis-Menten enzyme with the same Vmax? 4. Explain how pH and temperature influence enzyme activity. ( A Lineweaver-Burk (double reciprocal) plot was used to compare the effects of three different reversible inhibitors (A, B and C) on an enzyme. The plot of 1/V vs 1/[S] for the enzyme with no inhibitor is shown in a solid black line. The plot of 1/V vs 1/[S]...