When scraping and transferring the E.coli bacteria from the E.coli starter plate to the +BLU and...
When scraping and transferring the E.coli bacteria from the E.coli starter plate to the +BLU and -BLU tubes, why is it importnat that you not scrap up any of hte agar in the process?
Homework Answers
Answer:
The agar is the media for the bacteria to grow. if agar is scarped with culture, it may lead to impurities.During the transformation, side the loop smoothly over the surface to pick up the bacteria. You don't need tons of bacteria, but get enough so that you can see a small clump sticking to the loop.
During the transformation process, the bacteria is transformed to plasmid tubes using a loop. It is important not to transfer any agar medium along with bacteria bacause the impurities present in agar may interrupt the process of transformation or agar may itself act as an impurity and inhibit the transfrmation process.
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