Homework Answers
Diameter of the DNA plasmid is
caliculated from the circumference of the plasmid.
Circumference (C) is the outermost boundry of the circular plasmid.
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biophysics
15. Suppose that a plasmid consists of a circular DNA molecule containing 10 base a...
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circul...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
Draw a DNA molecule containing four base pairs. Identify the 5' end and the 3' end...
Draw a DNA molecule containing four base pairs. Identify the 5' end and the 3' end of each strand. Identify the Hydrogen bonds that hold the molecule together.
A relaxed B-type double stranded circular DNA molecule of 8,400 base pairs was cleaved by an...
A relaxed B-type double stranded circular DNA molecule of 8,400 base pairs was cleaved by an E. coli Type II topoisomerase. What was the original linking number (Lko) and the final linking number (Lk) for this DNA molecule? a. Lko=1,200; Lk=1,199 b. Lko=800; Lk=802 c. Lko-400; Lk=401 d. Lko+800; Lk=798 e. Lko=400; Lk=399 An explanation of the answer would be appreciated, thanks! The rate of bp/turn = 10.5.
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see...
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see on the gel? 2. How would you estimate the total number of base pairs in a plasmid by looking at the DNA fragments of the digested plasmid on a gel? 3. If a linear 1kb DNA fragment has a restriction site that is located 50 bp from one end of the plasmid, what would you expect to see if the digested and undigested DNA...
You are given a circular DNA molecule to analyze that is 3,000bp (3 Kb) long. You...
You are given a circular DNA molecule to analyze that is 3,000bp
(3 Kb) long. You proceed to treat the DNA molecule with different
cutting enzymes and then you run the individual reactions on a gel.
The following gel is produced with each column representing a
different reaction (lane). In lane 1 a molecule weight ladder is
run. In lane 2, the circular DNA is NOT treated with any enzyme. In
lane 3 the DNA is treated with an enzyme...
The average molecular weight of a nucleotide (base + deoxyribose + 1 phosphate group) in DNA...
The average molecular weight of a nucleotide (base + deoxyribose + 1 phosphate group) in DNA is 308g/mol of 308 Daltons (Da). This number is the average molecular weight of A = 312.2 g/mol G = 328.2 g/mol C = 288.2 g/mol T = 303.2 g/mol 55 R-plasmid was used for the transformation experiment a.What is the size of this plasmid (in kilo base pairs; kbp)? How many bases is this? b. What is the molecular weight of one plasmid...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
If 50.0 % of the base pairs in a duplex DNA molecule (12.5 kbp) are in...
If 50.0 % of the base pairs in a duplex DNA molecule (12.5 kbp) are in the B-conformation and 50.0 % are in the Z-conformation, what is the long dimension of the molecule?
[References If 34.0% of the base pairs in a duplex DNA molecule (12.5 kbp) are in...
[References If 34.0% of the base pairs in a duplex DNA molecule (12.5 kbp) are in the B-conformation and 66.0 % are in the Z-conformation, what is the long dimension of the molecule?
How many hydrogen bonds will be involved in base pairing in a DNA molecule of 50...
How many hydrogen bonds will be involved in base pairing in a DNA molecule of 50 base pairs that contains 15 cytosine bases? You Answered • 45 100 Correct Answer 115 135 150
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
Draw a DNA molecule containing four base pairs. Identify the 5' end and the 3' end of each strand. Identify the Hydrogen bonds that hold the molecule together.
You are given a circular DNA molecule to analyze that is 3,000bp
(3 Kb) long. You proceed to treat the DNA molecule with different
cutting enzymes and then you run the individual reactions on a gel.
The following gel is produced with each column representing a
different reaction (lane). In lane 1 a molecule weight ladder is
run. In lane 2, the circular DNA is NOT treated with any enzyme. In
lane 3 the DNA is treated with an enzyme...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
If 50.0 % of the base pairs in a duplex DNA molecule (12.5 kbp) are in the B-conformation and 50.0 % are in the Z-conformation, what is the long dimension of the molecule?
[References If 34.0% of the base pairs in a duplex DNA molecule (12.5 kbp) are in the B-conformation and 66.0 % are in the Z-conformation, what is the long dimension of the molecule?
How many hydrogen bonds will be involved in base pairing in a DNA molecule of 50 base pairs that contains 15 cytosine bases? You Answered • 45 100 Correct Answer 115 135 150
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