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Question 2 (1 point) In order to target a protein to the endomembrane system, which of the following is required first? O a EA tRNA is chemically modified so that the amino acid bound is different than the one specified by its anticodon. Which codonQuestion 6 (1 point) Transcribe and translate the following DNA sequence (coding strand): 5- ATGGTCGGTTATTAAGCA-3 Note - yoImagine if there were 1000 commonly occurring amino acids instead of 20. Given what you know about the genetic code, what wouQuestion 8 (1 point) A gene composed of 900 nucleotides could result in a protein containing a maximum of how many amino acidQuestion 9 (1 point) The codon within a particular mRNA molecule is complementary to its corresponding tRNA anticodon. cataly

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Answer #1

Ans: Correct option: Signal peptide on the N terminus of the polypeptide

Explanation: After synthesis, protein is first targeted to the endoplasmic reticulum. To target the protein into the endoplasmic reticulum a signal is required which is called targeting signal. This signal is located on the N-terminus and made up of the hydrophobic amino acid sequence and some basic amino acids.

Incorrect options:  

an ER bound ribosome

Explanation: when the protein that is synthesized by the ribosome has an ER specific signal sequence then the ribosome bind to the endoplasmic reticulum. Ribosome synthesizes proteins and they have no role in protein targeting to the endomembrane system.

Chaperon protein:

Explanation: These proteins help in the proper folding of the protein. They are required for protein targeting.

Signal peptide on the C-terminus of the polypeptide

Explanation: Signal peptide is present on the N-terminus and not on the C-terminus.

Signal recognition particle

Explanation:

  • As soon as the signal sequence comes out from the ribosome the signal recognition particle recognizes the signal sequence.
  • Then translation is temporarily stopped until the ribosome attaches to the SRP receptor (signal recognition particle receptor) present on the endoplasmic reticulum.
  • Now binding of GTP cause the transfer of signal sequence to the translocon and GTP hydrolysis cause the dissociation of the signal recognition particle.

Ans: Correct Answer: aminoacyl t-RNA synthetase will lose control over the amino acid

Explanation: Aminoacyl t-RNA synthetase is the enzyme that is responsible for the charging of the tRNA. That is the addition of the proper amino acid on to the t-RNA according to the anticodon present on the t-RNA. This enzyme will not work properly upon chemical modification of the tRNA as per the above-mentioned way.

Incorrect options:

Anticodon will match with the modified amino acid it carries

Explanation: As the t-RNA is chemically modified in such a way that amino acid is not as per the information provided by anticodon.

Anticodon will lose their specificity for t-RNA

Explanation:

Anticodons are the sequences present in the tRNA that binds to the specific codons that are present on the mRNA.

The anticodon will match the codon on the mRNA

Explanation: As the t-RNA is chemically modified and the amino acid is not according to the anticodon information. This will cause the braking of the aminoacyl t-RNA bond.

Ans:6 Correct option is the last one

5' AUGGUCGGUUAUUAAGCA 3'

AUG codes for methionine symbol M

GUC codes for valine symbol V

GGU codes for Glycine symbol G

UAU codes for tyrosine symbol Y

UAA is a stop codon

coding strand is the strand that has a similar sequence to the mRNA only there will uracil in place of thymine. The mRNA sequence is in the 5' to 3' direction.

The second option is incorrect because in RNA thymine is not present.

The third option is incorrect because GGU codes for glycine not for cytosine

last is incorrect because the mRNA is in 5' to 3' direction not in 3' to 5'

  

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