Please name TWO essential features of plasmid cloning vectors.
Essential features of plasmid cloning vectors are:-
Histochemical identification of recombinant clones.
Single-stranded DNA production.
Bacteriophage promoters.
Bacterial plasmids often serve as cloning vectors. Describe the essential features of a plasmid vector. What are the advantages and applications of plasmids as cloning vectors?
Describe the features that distinguish plasmid, phage, lambda, BAC and YAC cloning vectors.
compare and contrast 8 different types of cloning vectors. 1. prokaryote bacterial plasmid 2. Yeast Eukaryotic plasmid 3. Bacteriophage Lambda 4. Cosmos 5. Bacteriophage P1 6 PACs 7 BACs 8 YACs
Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids?a) Bacterial plasmids are linear in natureb) They are single strandedc) Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this propertyd) They are not capable of replication in bacteria
List two key component parts of a plasmid required for use in recombinant DNA cloning
The plasmid pFR55 is a useful vehicle for the cloning of any DBA sequence in E. coli. A restriction map of pFR55 showing the restriction enzyme cutting sites is shown below. The plasmid can replicate I'm E. coli and carries the tetracycline resistance Tc and ampicillin resistance (Ap) genes for use as selectable markers in E. coli. a. you wish to insert a gene into the SalI site of pFR55. How would you select for E. Coli cells that have...
AS. (5 points). The APOBEC enzyme deaminates cytosine to formuracil in both RNA and DNA. This is part of a natural anti-virus system that our cells use to protect themselves (or, try to). How might the deamination of cytosines help to deactivate present and future copies of virus nucleic acids? A6. (5 points). People who have spinal muscular atrophy (SMA) as a result of SMN1 deletions have much less severe disease if they also have extra copies of SMN2. Why...
is it true that the plasmid of interest is difficult for cloning and purifying the protein of your own interest.
Which of the following characteristic is not present in a plasmid on a general basis?a) Multiple cloning site (MCS)b) Origin of replication (ori)c) Antibiotic resistance gened) Beta galactose genes
Question 4 C. Cloning and restriction enzyme digest Video aid 1: Plasmid cloning Video aid 2: Restriction enzyme digest analysis The PCR was a success and your target region of 440 bp in length has been amplified. You igate a short linker containing an Apal restriction enzyme site onto both ends of the PCR product, digest it with Apal, and clone it into the Apal site of the 5 kb plasmid diagrammed below Bamll 300 EcoRI 3400 1000 2000 5...