Why is it necessary to use a special DNA polymerase ( Taq polymerase) in PCR?
-
Why is it necessary to use a special DNA polymerase ( Taq polymerase) in PCR?
Homework Answers
Taq polymerase is extracted from a thermophilic bacteria Thermus aqaticus that use to grow at 80-degree centigrade. In PCR the first step is denturation that is carried out at 94 degree centigrade. As we all know enzymes are proteins that will get denature if temeperautre is very high. At 94 degree centigrade normal DNA polymerase get denatured and can not work. However Taq polymerase is resistant to high temeprtaure and is can function at high temeperuate. So to overcome this probelem enzymes that could function at high temeperute are used. This is the reason why specific DNA polymerase are used in PCR.
Add Answer to:
Why is it necessary to use a special DNA polymerase (
Taq polymerase) in PCR?
Why is a heat-stable DNA polymerase from a thermophilic bacterium (the Taq polymerase) used in the...
Why is a heat-stable DNA polymerase from a thermophilic bacterium (the Taq polymerase) used in the PCR rather than a DNA polymerase from E. coli or humans? PCR involves heating the reaction at the beginning of each cycle to separate the newly synthesized ds DNA into single strands so that they can act as templates for the next round. a. UsingTaq avoids having to add it afresh for each round of DNA replication. b.Taq allows a faster transcription c. Taq...
Some of the PCR techniques for DNA amplification are: Regular PCR Hot start PCR High-Fidelity PCR...
Some of the PCR techniques for DNA amplification are: Regular PCR Hot start PCR High-Fidelity PCR Immuno PCR. Real-time PCR ------------------------------------ 2. The following information about Taq DNA polymerase is/are correct. Taq DNA polymerase was discovered by Kary Mullis in 1983. The entire Taq DNA polymerase reaction (PCR) technique was bought by Perkin Elmer in the range of $120 million. Kary Mullis received a Nobel prize in 1993 for discovering Taq DNA polymerase. Taq DNA polymerase was isolated from Thermus aquaticus....
what would happen in a PCR if the taq polymerase used was not thermostable?
what would happen in a PCR if the taq polymerase used was not thermostable?
help Which of the following enzyme is essential in PCR? RNA primers taq- polymerase • RNA...
help
Which of the following enzyme is essential in PCR? RNA primers taq- polymerase • RNA polymerase reverse transcriptase ORNA dependent RNA polymerase
Select the components and equipment that would be necessary for doing PCR. select all that apply...
Select the components and equipment that would be necessary for doing PCR. select all that apply Sample containing the DNA to be amplified dNTPs (deoxyribonucleotide triphosphates) Taq polymerase (thermostable polymerase) RNA polymerase Restriction endonucleases Primers specific to the DNA sequence to be amplified Thermocycler
1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the...
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
What is the purpose in a PCR reaction for each of the following reagents? Taq Taq...
What is the purpose in a PCR reaction for each of the following reagents? Taq Taq buffer dNTPs Forward primer Reverse primer Genomic DNA What do you think would happen if you forgot to add your Reverse primer when you did a PCR?
Lab 1 - PCR Pre-lab discussion. Discuss the following among your table prior to beginning the...
Lab 1 - PCR Pre-lab discussion. Discuss the following among your table prior to beginning the lab. 1. Describe the overall goal of the polymerase chain reaction. 2. List the components that are required for a PCR reaction. 3. A special DNA polymerase called Taq is used in PCR. What is unique about this enzyme and why is it used in the PCR reaction? 4. We will be using genomic DNA as our template DNA in this PCR reaction. How...
25. Which is not a required reactant for a PCR reaction? Select one: a. Primer DNA...
25. Which is not a required reactant for a PCR reaction? Select one: a. Primer DNA b. Taq polymerase c. dNTP d. template DNA e. ATP
Which of the following is NOT true about Taq DNA polymerase? Select one: a. It can...
Which of the following is NOT true about Taq DNA polymerase? Select one: a. It can act as an exonuclease to cut single-stranded DNA b. It adds nucleotide triphosphates to form a new DNA strand c. It functions at extremely high temperatures d. It requires a salt environment (buffer) to work
help
Which of the following enzyme is essential in PCR? RNA primers taq- polymerase • RNA polymerase reverse transcriptase ORNA dependent RNA polymerase
1.The PCR (polymerase chain reaction) protocol that is currently
used in laboratories was facilitated by the discovery of a
bacterium called Thermus aquaticus in a hot spring inside
Yellowstone National Park, in Wyoming. This organism contains a
heat-stable form of DNA polymerase known as Taq
polymerase, which continues to function even after it has been
heated to 95°C.
a.Why would such a heat-stable polymerase be beneficial in
PCR?
b.What would happen if it weren’t
heat stable?
c.How might you choose...
Lab 1 - PCR Pre-lab discussion. Discuss the following among your table prior to beginning the lab. 1. Describe the overall goal of the polymerase chain reaction. 2. List the components that are required for a PCR reaction. 3. A special DNA polymerase called Taq is used in PCR. What is unique about this enzyme and why is it used in the PCR reaction? 4. We will be using genomic DNA as our template DNA in this PCR reaction. How...
Most questions answered within 3 hours.
-
Why has California been the favorite destination of large number
of secondary migrants?
asked 10 minutes ago -
Do not neglect the old for the new. The existing business must
not lose priority simply...
asked 3 hours ago -
Kylie is a single mom with two dependent children,
Tanner, age 7 and Olivia, age 11....
asked 4 hours ago -
Phosphorous + bromine = phosphorous tribromide. If 35.0 g of
bromine are reacted and 27.9 grams...
asked 5 hours ago -
Derive the long wavelength limit of the Planck energy density
distribution
asked 5 hours ago -
Calculate the pH of each of the following solutions.
0.50 M HBr
3.1×10−4 M KOH
4.2×10−5...
asked 9 hours ago -
For the year ended December 31, Depot Max’s cost of merchandise
sold was $85,600. Inventory at the...
asked 9 hours ago -
Week 10 - Professional Memo Assignment
Professional Memo Assignment
Your mission for this week, should you...
asked 9 hours ago -
Write a Python program that stores the data for each
player on the team, and it...
asked 9 hours ago -
In
the last 3 months, mike never knows when he is going to get his
allowance...
asked 10 hours ago -
Is Ca(OH)2 a Bronsted base, Lewis base, or both? Why?
asked 9 hours ago -
1A- Why don’t voters complain about U.S. tariffs on imported
sugar?
Because sugar is only a...
asked 10 hours ago