Homework Answers
cDNA is better choice of nucleic acid(than DNA) because from those cDNA copies you can do things like PCR amplify a gene of interest, or make a cDNA library and probe it for a specific sequence or activity.
Eukaryote genes commonly contain introns (non-coding sequences). These are eliminated after mRNA synthesis so cDNA does not contains any introns. Which means that a cDNA copy of a gene can be isolated as a single, intron-free fragment. Prokaryotes does not contain introns so this is not a point to be considered when working with bacterial genes.
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.. Why is cDNA a better choice of nucleic acid (than DNA) when wanting to understand...
Part A -Why is cDNA used in RNAseq? Because cDNA is more stable than RNA Because...
Part A -Why is cDNA used in RNAseq? Because cDNA is more stable than RNA Because it captures all the genes expressed in a tissue or a cell Existing sequencing protocols are designed for sequencing DNA All the above are true Submit Request Answer Part B-Why do scientists measure gene expression? Because Gene expression is static, and the same gene is always expressed the same Because Gene expression realtes to changes in the genomic DNA Because Gene expression is dynamic,...
Q14: In order to express a human protein in a bacterium, the complementary DNA (cDNA) of...
Q14: In order to express a human protein in a bacterium, the complementary DNA (cDNA) of that human gene must be inserted into the bacterium rather than simply the gene cut out of genomic DNA. Please explain why.
You characterize the sequence of a full-length cDNA and the corresponding genomic DNA for a particular...
You characterize the sequence of a full-length cDNA and the corresponding genomic DNA for a particular intron-containing gene from mouse cells. When you align them to each other using a computer program, the exons of the cDNA align perfectly in some regions with pieces of the genomic DNA, whereas other exons appear to have a small number of specific nucleotide differences compared to the genomic DNA. Assume this genomic DNA and cDNA come from the same individual, and there are...
14.11 The Organic Chemistry of Biomolecules: Aromaticity and DNA 14.48 A strand of nucleic acid is...
14.11 The Organic Chemistry of Biomolecules: Aromaticity and DNA 14.48 A strand of nucleic acid is defined by its sequence of nucleotides: A, C, T, and G. How many different sequences are possible for a nucleic acid that is 200 nucleotides long? How does that number compare to the estimated number of atoms in the universe, which is approximately 1080? CHAPTER 14 Orbital Interactions 2 720
14.11 The Organic Chemistry of Biomolecules: Aromaticity and DNA 14.48 A strand of nucleic...
A specific stretch of DNA that programs the amino acid sequence of a protein is a...
A specific stretch of DNA that programs the amino acid sequence of a protein is a A. nucleic acid B. protein C. gene D. enzyme
Why is CD Spectroscopy a useful tool for characterizing nucleic acid structures? Why are nucleic acids...
Why is CD Spectroscopy a useful tool for characterizing nucleic acid structures? Why are nucleic acids perfectly applicable for CD analyses? Name and describe another instrumental method for charactering the structure of DNA and RNA?
MICROBIAL ECOLOGY: The next set of methods refers to nucleic acid probes. What are these, and...
MICROBIAL ECOLOGY: The next set of methods refers to nucleic acid probes. What are these, and how do they work? Select all that are true The term refers to DNA or RNA oligonucleotide complementary to a sequence in a target gene. The probe can be used to identify organisms containing a nucleic acid sequence complementary to the probe. Oligonucleotide probes can be made fluorescent with certain dyes. The probes can be used in the method called FISH.
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Given the following diagram of a typical eukaryotic coding gene, draw a basic sketch showing gene...
Given the following diagram of a typical eukaryotic coding gene, draw a basic sketch showing gene transcription and processing into a mature mRNA. a. Given your answer to question 1, would you expect a typical mRNA (or its derived cDNA) sequence to completely align with a genomic sequence in a single contiguous location? (i.e., the mRNA sequence aligns without breaks to the genomic sequence)? Explain. Why is it easier to predict the location of genes in microbial genomes than in...
When binary fission occurs, name the type of nucleic acid much information is copied. of nucleic...
When binary fission occurs, name the type of nucleic acid much information is copied. of nucleic acid involved and how What is the difference between binary fission and protein synthesis: What is meant by "complementarity of bases? What are nucleotide bases? Name the nucleotide bases associated with DNA with RNA. How many nucleotide bases are necessary to code for one amino acid? Name the two different classes of genes. Structural Regulaory What are constitutive enzymes? What are inducible and repressible...
Part A -Why is cDNA used in RNAseq? Because cDNA is more stable than RNA Because it captures all the genes expressed in a tissue or a cell Existing sequencing protocols are designed for sequencing DNA All the above are true Submit Request Answer Part B-Why do scientists measure gene expression? Because Gene expression is static, and the same gene is always expressed the same Because Gene expression realtes to changes in the genomic DNA Because Gene expression is dynamic,...
14.11 The Organic Chemistry of Biomolecules: Aromaticity and DNA 14.48 A strand of nucleic acid is defined by its sequence of nucleotides: A, C, T, and G. How many different sequences are possible for a nucleic acid that is 200 nucleotides long? How does that number compare to the estimated number of atoms in the universe, which is approximately 1080? CHAPTER 14 Orbital Interactions 2 720
14.11 The Organic Chemistry of Biomolecules: Aromaticity and DNA 14.48 A strand of nucleic...
Scientists have engineered bacteria to produce human proteins such as human growth hormone (hGH) to help treat dwarfism. Suppose the unmodified eukaryotic gene coding for hGH is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the hGH gene. Bacteria cannot remove intronic sequence from a gene, so if the gene for hGH were transcribed, it would translate to a nonfunctional protein. The bacterial nucleoid does not have the...
Given the following diagram of a typical eukaryotic coding gene, draw a basic sketch showing gene transcription and processing into a mature mRNA. a. Given your answer to question 1, would you expect a typical mRNA (or its derived cDNA) sequence to completely align with a genomic sequence in a single contiguous location? (i.e., the mRNA sequence aligns without breaks to the genomic sequence)? Explain. Why is it easier to predict the location of genes in microbial genomes than in...
When binary fission occurs, name the type of nucleic acid much information is copied. of nucleic acid involved and how What is the difference between binary fission and protein synthesis: What is meant by "complementarity of bases? What are nucleotide bases? Name the nucleotide bases associated with DNA with RNA. How many nucleotide bases are necessary to code for one amino acid? Name the two different classes of genes. Structural Regulaory What are constitutive enzymes? What are inducible and repressible...
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