Question

Regulation of Gene Expression Describe the process of cell determination and differentiation in a future skeletal muscle cell.

Answer 1

The determination of different cell types involves progressive restrictions in their developmental potentials. when a cell "chooses" a particular fate, it is said to be determined ,although it still "looks" just like its undetermined neighbors.determination implies a stable change the fate of determined cells does not change.

Differentiation follows determination,as the cell elaborates a cell specific developmental program .differentiation results in the presence of cell types that have clear cut identies , such as muscle cells ,nerve cells, and skin cells.

Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation Two types of stem cells in skeletal muscle can differentiate into osteogenic cells:muscle derived stem cells,and bone marrow derived stem cells in the blood. in the present study we transferred the BMP gene into rat skeletal muscles,we then stained tissues for several muscle derived stem cell markers,muscle regeneration related markers, and an inflammatory cell marker to follow cell differentiation oer time.the results revealed that commitment to osteogenic differentiation was determined with in ten days of BMP gene transfer and consequent muscle regeneration.skeletal muscle has high potential for self regeneration.