1- In addition to the DNA building blocks (nucleotides dATP, dCTP, dGTP, dTTP) the master mix...
1- In addition to the DNA building blocks (nucleotides dATP, dCTP, dGTP, dTTP) the master mix contain:
A) Taq DNA polymerase
B) Buffer
C) Magnesium chloride
D) Specific primers to amplify the BXPOO7 locus
E) All of the above
2- PCR partially simulates ‘in vivo”
A) RNA synthesis
B) DNA transcription to RNA
C) Protein synthesis
D) DNA replication
Homework Answers
E) All of the above . Taq DNA polymerase will polymerize the nucleotides , buffer will provide optimum pH conditions for the enzyme to work , MgCl2+ act as a cofactor for Taq polymerase and primers will help in specific amplification of BXPOO7 .
2) DNA replication . Beacuse new copy of a specific DNA sequence is generated in vitro.
Add Answer to:
1- In addition to the DNA building blocks (nucleotides dATP,
dCTP, dGTP, dTTP) the master mix...
Suppose you are trying to synthesize DNA molecules In test tubes in the laboratory. A single...
Suppose you are trying to synthesize DNA molecules In test tubes in the laboratory. A single stranded DNA molecule with the sequence 3'- GCGCGGAGTCCCTATCTGCA-5' will be used as the template strand for DNA synthesis. You also have at your disposal an DNA primer with the sequence 5'- CGCGCC-3', a DNA polymerase, and nucleotides You perform Iive separate experiments using the nucleotides indicated below: Experiment 1: dATP, dCTP, dGTP, and dTTP Experiment 2 dATP, dCTP. GTP, and dTTP Experiment 3 dATP,...
If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...
If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you predict them to be? d. O PCR Master Mix GoTaq DNA Polymerase 2.5 μM forward primer: 5'-AACTGGCAGAATAAAGATCTC 2.5 ㎂Λ reverse primer: 5-AACACAAACCATCACSCCTATTTT-3 200 μΜ dATP, dGTP, dCTP, dTTP 1.5 mM MgCl2. Nuclease free H,O
4 total reactions 10. Complete this Master Mix table for 3 DNA samples, a positive control,...
4 total reactions
10. Complete this Master Mix table for 3 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. 4 pts) Master C Mix Conc. Of Final Stock solution L/Rxn Number of Total L (total per Reactions needed tube) for master mix PCR buffer Water dNTP mix MgCl2 F Primer 20X 1X | | 25 mM2.5 mM 10uM | 0.1 μΜ 100 ml 100 μΜ RPrimer | 10uM | 0.1μΜ Taq...
11. A gene is best defined as a. A segment of DNA b. Three nucleotides that...
11. A gene is best defined as a. A segment of DNA b. Three nucleotides that code for an amino acid. C. A sequence of nucleotides in DNA that codes for a functional product. d. A sequence of nucleotides in RNA that codes for a functional product. e. A transcribed unit of DNA. 12. Which of the following statements is false? a. DNA polymerase joins nucleotides in one direction only. b. The leading strand of DNA is made continuously c....
Transcription is the process of rewriting DNA. DNA molecules are made from 4 different nucleotides which...
Transcription is the process of rewriting DNA. DNA molecules are made from 4 different nucleotides which acts as building blocks. These building blocks are adenine, thymine, guanine and cytosine. When put together in different chemical combinations they become directions for the functions of the cell molecules which are primarily proteins. When a certain protein is needed, the RNA polymerase enzyme will find the gene for that particular protein and makes an RNA copy of it. DNA and RNA have similar...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
The building blocks (monomers) of DNA are: 1) amino acids O2) histones O 3) nucleotides O4)...
The building blocks (monomers) of DNA are: 1) amino acids O2) histones O 3) nucleotides O4) mutations 5) DNA polymerase 06) single nucleotide polymorphisms (SNPs)
Exercise 2: Heredity and DNA replication 1. In Mendel’s experiments with pea plants, name and describe...
Exercise 2: Heredity and DNA replication 1. In Mendel’s experiments with pea plants, name and describe the experiment which demonstrated that emasculation was not the reason why the wrinkled seed shape disappeared in the F1 generation. (4 marks) 2. In Mendel’s experiments with pea plants, test crosses used a plant which was homozygous recessive to determine the genotype of the F2 plant. Why did the test crosses not use plants which was homozygous for the dominant allele? (2 marks) 3....
Directions: Read through each question thoroughly. through your assignment tab. Highlight the correct answer. Submit the quiz 1. Which enzymes are responsible for adding nucleotides to the DNA mo...
Directions: Read through each question thoroughly. through your assignment tab. Highlight the correct answer. Submit the quiz 1. Which enzymes are responsible for adding nucleotides to the DNA molecule during DNA replication? a) DNA replicase b) DNA polymerase c) DNA catalase d) DNA gyrase 2. A bacterial cell with faulty RNA polymerase would not be able to carry out a) DNA replication b) double helix formation c) DNA supercoiling d) transcription 3. R plasmids are of particular concern to humans...
24. What would be the anticodon if the template strand of DNA Is ACC A UCC...
24. What would be the anticodon if the template strand of DNA Is ACC A UCC B.) TGG UGG D. ACC E. TCC 25. Prior to protein synthesis, the DNA A. attracts tRNAs with appropriate amino acids. 6.) serves as a template for the production of mRNA. C. adheres to ribosomes for protein synthesis. D. contains anticodons that become codons. E. must first undergo replication. 26. The Human Genome Project has revealed that human DNA has approximately A. 30,000 bases...
Suppose you are trying to synthesize DNA molecules In test tubes in the laboratory. A single stranded DNA molecule with the sequence 3'- GCGCGGAGTCCCTATCTGCA-5' will be used as the template strand for DNA synthesis. You also have at your disposal an DNA primer with the sequence 5'- CGCGCC-3', a DNA polymerase, and nucleotides You perform Iive separate experiments using the nucleotides indicated below: Experiment 1: dATP, dCTP, dGTP, and dTTP Experiment 2 dATP, dCTP. GTP, and dTTP Experiment 3 dATP,...
If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you predict them to be? d. O PCR Master Mix GoTaq DNA Polymerase 2.5 μM forward primer: 5'-AACTGGCAGAATAAAGATCTC 2.5 ㎂Λ reverse primer: 5-AACACAAACCATCACSCCTATTTT-3 200 μΜ dATP, dGTP, dCTP, dTTP 1.5 mM MgCl2. Nuclease free H,O
4 total reactions
10. Complete this Master Mix table for 3 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. 4 pts) Master C Mix Conc. Of Final Stock solution L/Rxn Number of Total L (total per Reactions needed tube) for master mix PCR buffer Water dNTP mix MgCl2 F Primer 20X 1X | | 25 mM2.5 mM 10uM | 0.1 μΜ 100 ml 100 μΜ RPrimer | 10uM | 0.1μΜ Taq...
11. A gene is best defined as a. A segment of DNA b. Three nucleotides that code for an amino acid. C. A sequence of nucleotides in DNA that codes for a functional product. d. A sequence of nucleotides in RNA that codes for a functional product. e. A transcribed unit of DNA. 12. Which of the following statements is false? a. DNA polymerase joins nucleotides in one direction only. b. The leading strand of DNA is made continuously c....
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
The building blocks (monomers) of DNA are: 1) amino acids O2) histones O 3) nucleotides O4) mutations 5) DNA polymerase 06) single nucleotide polymorphisms (SNPs)
Directions: Read through each question thoroughly. through your assignment tab. Highlight the correct answer. Submit the quiz 1. Which enzymes are responsible for adding nucleotides to the DNA molecule during DNA replication? a) DNA replicase b) DNA polymerase c) DNA catalase d) DNA gyrase 2. A bacterial cell with faulty RNA polymerase would not be able to carry out a) DNA replication b) double helix formation c) DNA supercoiling d) transcription 3. R plasmids are of particular concern to humans...
24. What would be the anticodon if the template strand of DNA Is ACC A UCC B.) TGG UGG D. ACC E. TCC 25. Prior to protein synthesis, the DNA A. attracts tRNAs with appropriate amino acids. 6.) serves as a template for the production of mRNA. C. adheres to ribosomes for protein synthesis. D. contains anticodons that become codons. E. must first undergo replication. 26. The Human Genome Project has revealed that human DNA has approximately A. 30,000 bases...
Most questions answered within 3 hours.
-
A beta (β) particle is described by which of the following?
Select the correct answer below:...
asked 17 seconds from now -
A new machine costs $150,000, lasts 10 years, has an annual
O&M cost of $50,000, and...
asked 2 minutes ago -
A newspaper in a large Midwestern city reported that the
National Association of Realtors said that...
asked 4 minutes ago -
1. Develop a use case diagram for the bank case study described
in Chapter 1. 2....
asked 14 minutes ago -
The phylum Protista has often been referred to as a junk drawer
of classification. Explain what...
asked 20 minutes ago -
The average amount of money spent for lunch per person in the
college cafeteria is $7.09...
asked 23 minutes ago -
You follow the lab procedure and at the end of Part 4C, you used
an average...
asked 41 minutes ago -
Write 250-450 words, about the following: • Analyze how a
servant leader in your organization or...
asked 45 minutes ago -
3. American and Japanese workers can each produce 4 cars a
year. An American worker can...
asked 48 minutes ago -
On October 1, 20X1, a company purchased a piece of land by
agreeing to pay the...
asked 1 hour ago -
Jamie is doing a survey at her school about whether the students
feel the cafeteria food...
asked 1 hour ago -
What are the roles of 3' and 5' untranslated regions in
mRNAs?
asked 1 hour ago