To calculate 10 ug of RNA by gel electrophoresis, how much of your prep would you need to load on the gel?
For RNA gel electrophoresis we use 15ug of protein in 4ul of sample solution.
So, 10 ul of RNA present in 6ul of sample.
Detail procedures are attached below-
To calculate 10 ug of RNA by gel electrophoresis, how much of your prep would you...
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
. In preparation of gel electrophoresis, how many grams of SDS do you need to prepare for 2 grams of protein?
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
If you stopped gel electrophoresis halfway during the running time, what would you expect? not all the fragments have separated from each other in the gel yet the smallest fragments will still be in the loading well of the gel the largest fragments will be at the opposite end of the gel
At the end of gel electrophoresis, what would you expect to find? the largest fragments closest to the wells O the mid-sized fragments toward the middle of the gel the smallest fragments toward the opposite end of the gel from the loading wells O all of the above
Stuck answering the rest of these 3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
a) Calculate how you would make 1.0 L of 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW=121.4 g/mole; Boric Acid MW=61.84 g/mole; EDTA MW=292.2 g/mole b) For your polyacrylamide gel electrophoresis gel you need 50 mL of 0.5x TBE. Calculate and describe how you would make this solution using your stock solution.
Do not calculate the solution to this problem but show how it would be set up. Assume you are trying to create a gel for gel electrophoresis that had a 1.2% concentration of agarose. If you had 50X TBE as the liquid buffer and the gel size was 20mL, how much 50X TBE would you need? How much water? Receptors in the medulla of the brain will stimulate respiration when hydrogen ion concentration increases. Normal blood pH is 7.4. If...
• What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: • (Allele 1: 3 TAGA repeats) • (Allele 2: 6 TAGA repeats) -----------TAGATAGATAGA----------- -----------TAGATAGATAGA TAGATAGATAGA--- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on...