DNA polymerases I, III, and delta have important roles in replication. Which of these proteins have similar functions? Describe those similar functions that they share. Which one performs a different function and what is that function?
Ans) DNA polymerase-I,III and delta has 3' ---> 5' exonuclease activity for the removal of RNA primer wheras DNA polymerase-I (small subunit) also has 5' -->3' exonuclease activity.
DNA polymerase-I,III, and delta can synthesize DNA from 5' --> 3' end.
DNA polymerase-I is used mainly for short fragment of DNA synthesis wheras DNA-Polymerase III is main replicating enzyme and has high catalytic processivity and high catalytic efficiency. DNA pol delta also has high processivity.
DNA polymerases I, III, and delta have important roles in replication. Which of these proteins have...
32. Which statement is correct for biological DNA replication? Select one: O a. DNA polymerases always require a primer. b. It needs the single strand binding proteins. c. DNA replication starts at the Ori sequence. d. DnaA is a protein which steadily accumulates to trigger DNA replication i ee. All of these
1. DNA is coiled around what type of proteins to form nucleosomes A. Polymerases DNA replication of the lagging strand is discontinuous B. Transcription factors DNA replication of the lagging strand is continuous C. Helicases D. Histones E. DICER 2. Which of the following statements is true? A. DNA replication of the leading strand is discontinuous B. DNA replication of the lagging strand is discontinuous C. DNA replication of the leading strand is dispersive D. DNA replication of the lagging...
Which statement is correct for biological DNA replication? Select one: a. DNA polymerases always require a primer. b. It needs the single strand binding proteins. c. DNA replication starts at the Ori sequence. d. DnaA is a protein which steadily accumulates to trigger DNA replication in E.coli. e. All of these
Briefly discuss the roles of the key enzymes and proteins involved in DNA replication in E.coli. In conclusion to your answer, describe the arrangement of these factors in the holenzyme complex at the E.coli DNA replication fork
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in the replication of E. coli DNA. What features of the E. coli replisome and of DNA polymerase III’s structure are associated with replication processivity? How do mammalian cells prime discontinuous strand replication and how do they remove RNA primers?
SELLUN 9. Contrast the functions of DNA polymerase III and DNA polymerase I. 10. Why do are the start points of DNA replication in eukaryotic and prokaryotic organisms AT rich? 11. What is the start point of DNA replication called in E. coli? What is it called in humans? 12. In DNA replication in E. coli which enters the replication fork first, DnaA protein, or Helicase? 13. In eukaryotes, two proteins are involved in linking DNA replication to S phase...
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the function of Helicase, Primase, topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase
RNA polymerases lack proofreading ability. Define proofreading ability and describe its importance in replication of DNA genomes. a. What advantage might its absence give RNA viruses like SARS-CoV-2? Following protein synthesis, RNA genome and proteins assemble to form virus particles that then bud into the endoplasmic reticulum (ER) lumen and then on to the Golgi apparatus. Next, they are transported via Golgi vesicles to the cell membrane, where they are released via exocytosis. Define ER, Golgi apparatus, and exocytosis,...
1. During DNA replication the lagging strand is complemented with Okazaki fragments that still contain the RNA primer. Which pair of enzymes removes the RNA primer and seals the DNA nicks? DNA polymerase I and ligase the DNA polymerases II and III primase and gyrase DNA polymerase III and helicase DNA polymerase III and primase 2. Which of the following best describes the process of DNA replication in a prokaryote? Replication begins at multiple sites, spreading outward until the entire...