What is meant by the size of an allele in the PCR gel if each PCR...
What is meant by the size of an allele in the PCR gel if each PCR band represents an Allele?
Homework Answers
The alleles are giving bands in gel electrophoresis. The separation is on the basis of charge and size only. The larger the size, the less movement of the allele in the gel. In gel electrophoresis, we use ladder of our known size and we use ladder in one of the well. The bands on ladder at particular position shows the size of the relative bands. The ladder size generally starts from 100 bp to 3000 bp and it varies depending upon the size of the alleles. So here we can see the relative size of the allele with respect to ladder.
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What is meant by the size of an allele in the PCR gel if each
PCR...
• What would you expect to see on a gel if PCR is performed for a...
• What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: • (Allele 1: 3 TAGA repeats) • (Allele 2: 6 TAGA repeats) -----------TAGATAGATAGA----------- -----------TAGATAGATAGA TAGATAGATAGA--- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on...
What would you expect to see on a gel if PCR is performed for a microsatellite...
What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: (Allele 1: 3 TAGA repeats) (Allele 2: 6 TAGA repeats) ---TAGATAGATAGA------ ---TAGATAGATAGA TAGATAGATAGA-- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on the gel (or...
II. Amplify the gene of interest using the PCR, verify the PCR products on the gel...
II. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. 4. By the next morning, the PCR amplification of all the samples is over, and you set up gel electrophoresis to check for the PCR products. Results: Lane 1 Alexandra - one band Lane 2 Olga - one band Lane 3 Tatiana - one band Lane 4 Maria - one band Lane 5 Anastasia - one band Lane 6 Alexey...
DNA from 100 unrelated people was amplified by PCR with allele specific primers, The PCR products...
DNA from 100 unrelated people was amplified by PCR with allele specific primers, The PCR products were separated and visualized on the following gel. A specific fragment was found to correspond to each blood group allele, indicated on the left hand side. The number of individuals with each gel pattern is shown across the top. 7351-749, 18 Assuming random mating, what is the expected frequency of Type B blood? А 0.0025 0.070 C. 0.0725 D 0.25 E 0.05
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
Why is PCR required before running the DNA on a gel? A Without PCR, there would...
Why is PCR required before running the DNA on a gel? A Without PCR, there would be things in the DNA that would interfere with running it on the gel. B Without PCR, there would be too little of the DNA region of interest to see it on the gel. C Without PCR, the DNA region of interest would not be present because PCR adds the part that will be examined. D Without PCR, the gel would not have a...
Using PCR an RFLP was identified on the first chromosome of wild strains of Banana slugs...
Using PCR an RFLP was identified on the first chromosome of wild strains of Banana slugs isolated in Santa Cruz. In strain C, the fragment could be cut with EcoR1 yielding 3 kb and 4 kb bands when run on a gel. In strain E, the fragment was not cut by Eco1 resulting in a 7 kb band run on a gel. A strain E in lividual that is also homozygous for the first chron come recessive mutation day-glo (dg)...
Table 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA ...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
1. What is the purpose of the PCR nucleotide mix added to your PCR reaction? 2....
1. What is the purpose of the PCR nucleotide mix added to your PCR reaction? 2. What is the purpose of Gel Green? What type of chemical is it? Molecularly how does it work? 3. Why are the restriction digest reactions mixed by flicking or pipetting, rather than vortexing? 4. In the ligation, hAPP can only insert in one orientation into the vector why is this? What is the role of hAPP in DNA replication? What is its role in...
genetics there are many parts to this question each in a different photo Extended calculation problem...
genetics there are many parts to this question each in a different
photo
Extended calculation problem 1 Using PCR an RFLP was identified on the first chromosome of wild strains of Banana slugs isolated in Santa Cruz. In strain C, the fragment could be cut with EcoR1 yielding 3 kb and 4 kb bands when run on a gel. In strain E, the fragment was not cut by EcoR1, resulting in a 7 kb band run on a gel. A...
• What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: • (Allele 1: 3 TAGA repeats) • (Allele 2: 6 TAGA repeats) -----------TAGATAGATAGA----------- -----------TAGATAGATAGA TAGATAGATAGA--- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on...
What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: (Allele 1: 3 TAGA repeats) (Allele 2: 6 TAGA repeats) ---TAGATAGATAGA------ ---TAGATAGATAGA TAGATAGATAGA-- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on the gel (or...
II. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. 4. By the next morning, the PCR amplification of all the samples is over, and you set up gel electrophoresis to check for the PCR products. Results: Lane 1 Alexandra - one band Lane 2 Olga - one band Lane 3 Tatiana - one band Lane 4 Maria - one band Lane 5 Anastasia - one band Lane 6 Alexey...
DNA from 100 unrelated people was amplified by PCR with allele specific primers, The PCR products were separated and visualized on the following gel. A specific fragment was found to correspond to each blood group allele, indicated on the left hand side. The number of individuals with each gel pattern is shown across the top. 7351-749, 18 Assuming random mating, what is the expected frequency of Type B blood? А 0.0025 0.070 C. 0.0725 D 0.25 E 0.05
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
Using PCR an RFLP was identified on the first chromosome of wild strains of Banana slugs isolated in Santa Cruz. In strain C, the fragment could be cut with EcoR1 yielding 3 kb and 4 kb bands when run on a gel. In strain E, the fragment was not cut by Eco1 resulting in a 7 kb band run on a gel. A strain E in lividual that is also homozygous for the first chron come recessive mutation day-glo (dg)...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
genetics there are many parts to this question each in a different
photo
Extended calculation problem 1 Using PCR an RFLP was identified on the first chromosome of wild strains of Banana slugs isolated in Santa Cruz. In strain C, the fragment could be cut with EcoR1 yielding 3 kb and 4 kb bands when run on a gel. In strain E, the fragment was not cut by EcoR1, resulting in a 7 kb band run on a gel. A...
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