Question

Translate the following E.coli mRNA into a polypeptide. Translate the following human mRNA into a polypeptide.

Answer 1

Question 1: UCUAUGGAGGAGGAUCGAUUAUGCAAGCCUUUACGUAAGCUUACGC

We can use Translate tool from Expasy (http://web.expasy.org/translate/). This tool shows all six possible combinations.

5'3' Frame 1
tct atg gag gag gat cga tta tgc aag cct tta cgt aag ctt acg c

 S M  E  E  D  R  L  C  K  P  L  R  K  L  T      

5'3' Frame 2
tctatggaggaggatcgattatgcaagcctttacgtaagcttacgc

  L  W  R  R  I  D  Y  A  S  L  Y  V  S  L  R  

5'3' Frame 3
tctatggaggaggatcgattatgcaagcctttacgtaagcttacgc

   Y  G  G  G  S  I  M  Q  A  F  T  -  A  Y    

3'5' Frame 1
gcgtaagcttacgtaaaggcttgcataatcgatcctcctccataga

 A  -  A  Y  V  K  A  C  I  I  D  P  P  P  -    

3'5' Frame 2
gcgtaagcttacgtaaaggcttgcataatcgatcctcctccataga

  R  K  L  T  -  R  L  A  -  S  I  L  L  H  R  

3'5' Frame 3
gcgtaagcttacgtaaaggcttgcataatcgatcctcctccataga

   V  S  L  R  K  G  L  H  N  R  S  S  S  I    

Since the first amino acid should be methionine (M) the correct frame is 5'3' Frame 1 and the sequence of amino acids is M E E D R L C K P L R K L T

Question 2: UACGAGUAAGUCAGUGACAGAUGGAGGAUAUGUGAUCAUAGC

Using Translate tool

5'3' Frame 1

tacgagtaagtcagtgacagatggaggatatgtgatcatagc

 Y  E  -  V  S  D  R  W  R  I  C  D  H  S  

5'3' Frame 2
tacgagtaagtcagtgacagatggaggatatgtgatcatagc

  T  S  K  S  V  T  D  G  G  Y  V  I  I    

5'3' Frame 3
tacgagtaagtcagtgacagatggaggatatgtgatcatagc

   R  V  S  Q  -  Q  M  E  D  M  -  S  -    

3'5' Frame 1
gctatgatcacatatcctccatctgtcactgacttactcgta

 A  M  I  T  Y  P  P  S  V  T  D  L  L  V    

3'5' Frame 2
gctatgatcacatatcctccatctgtcactgacttactcgta

  L  -  S  H  I  L  H  L  S  L  T  Y  S    

3'5' Frame 3
gctatgatcacatatcctccatctgtcactgacttactcgta

   Y  D  H  I  S  S  I  C  H  -  L  T  R   

So the correct frame is 3'5' Frame 1 and the amino acid sequence is  M I T Y P P S V T D L L V

Please use expasy yourself to learn more. All the best.