features of restriction enzyme include the following, except that they
Answer : are produced by both prokaryotes and eukaryotes
Restriction enzymes are produced only by the prokaryotes ( bacteria) to cleave foreign DNA at specific sites.
features of restriction enzyme include the following, except that they
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition BamHI G↓GATCC CCTAG↑G B) Enzyme Recognition EcoRI G↓AATTC CTTAA↑G C) Enzyme Recognition HaeIII GG↓CC CC↑GG D) Enzyme Recognition HindIII A↓AGCTT TTCGA↑A E) all of the above, except c
Features of Job order production include all of the following except Multiple Choice Diversity of products produced. Mass production. Heterogeneity. Customization Separate manufacturing from other products
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
A restriction digest of a circular plasmid is conducted using two different restriction enzymes. Restriction enzyme 1 cuts the plasmid in one place while restriction enzyme 2 cuts in two places. All restriction sites are at least 1kB apart from each other. Based on this information, the resulting gel should have _____ bands for the enzyme 1 reaction, _____ bands for the enzyme 2 reaction, and _____ bands for the reaction that contains both enzymes A 1; 2; 3 B...
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site of 5’GAATTC 3” and the cutting point between G and A. a. How many fragments you will get b. Specify the size (Number of bases) and the sequence of each fragment, pay attention to DNA direction (5’-3’) 5” ACATTGTCCGGGAATTC CGGGCTAGGCAT T GAATTGGAACA GAATTC GGGCCCGATCCGTA 3
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme Eco R1. A map of the plasmid is shown here. The entire plasmid is 6000 bp, and there are Eco R1 restriction sites at 1500 bp, 2000 bp, and 4000 bp. You’re going to run the entire volume of the digest on a gel, and you want to cut just enough DNA to have 50 ng in the smallest band on your gel. Starting...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
Which of these is the correct sequence of events? Group of answer choices: restriction enzyme digest-> heat inactivation->agarose gel analysis enzyme digest->heat inactivation heat inactivation->agarose gel analysis->restriction enzyme digest heat inactivation->restriction enzyme digest->agarose gel analysis
Of the following steps to make recombinant DNA, which step occurs immediately after a restriction enzyme recognizes specific nucleotide sequences?
EcoRI is a common restriction enzyme used in cloning experiments. Its restriction sequence is G’AATTC. The strand is cut at the position of the apostrophe. The 50 base pair sequence shown below contains one or more EcoRI sites. Find them and circle them. Then count the resulting size (in base pairs – bp) of the DNA fragments (pieces) left over after using EcoRI to cut this DNA sequence. Circle the EcoRI sites in the sequence below. 1- ggagaattcgctgtacgaggttaaccccgatgccATGGCATGAATTCGTG -50 List...