How many primers does it take to amplify a single PCR product? Explain
How many primers does it take to amplify a single PCR product? Explain
Homework Answers
Two primers are required to amplify a single pcr product. One primers attach or anneal with upper strand and 2nd anneal lower strand for amplifying DNA. Both will amplify in direction of 5' to 3'.
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How many primers does it take to amplify a single PCR product?
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In PCR, how does length/composition of primers affect annealing?
In PCR, how does length/composition of primers affect annealing?
Which of the following components is NOT used in the technique of PCR to amplify a...
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A....
EXPLAIN each answer thoroughly. There are two common goals when using PCR to amplify DNA. A. Make lots of copies of a specific DNA sequence to use in cloning (preparative PCR) B. Detect the presence or relative amount of a specific gene under varying conditions (analytical PCR) *Remember: PCR is very similiar to DNA replication, but uses a DNA polymerase to amplify only specific parts of DNA sequence based on the sequence of the primers. 3. For which goal (A...
Choose the two primers from the list that will amplify the region indicated by the bracket....
Choose the two primers from the list that will amplify the region indicated by the bracket. The PCR product should be 25 base pairs long. (The DNA has dotted lines every 5 base pairs to help with counting.) Choose TWO correct primers 4 points possible 5' ...TTTTGAAGGCCACACTTTCCCTCTCAAAGGCCCGGAACCC... 3' 3' ...AAAACTTCCGGTGTGAAAGGGAGAGTTTCCGGGCCTTGGG... 5' region to be amplified If image does not load, click here to access. 5'GGAAA 3 5'CCGGG 3 5'GAGAG 3 5'CACAC 3' a 5'CCTTT 3 n 5'TTTTG 3 5'TTCCG 3...
• 4. How many copies of the primers do you add to the PCR? You add...
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
1. Explain why, when PCR is used to amplify the same region of DNA from two...
1. Explain why, when PCR is used to amplify the same region of DNA from two different people, the size of the DNA fragment(s) generated may be different? 2. What characteristic of the DNA molecule makes it possible to use electrophoresis to separate DNA molecules by size? Explain why this characteristic is important for electrophoresis and what part of the DNA molecule creates this characteristic. 3. You are performing PCR. After four cycles of PCR, how many double-stranded copies of...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
You’re trying to PCR amplify a gene, but your gel shows that there are two bands...
You’re trying to PCR amplify a gene, but your gel shows that there are two bands instead of only one like you expected. You know for certain that there no homologous genes in the genome from which you’re amplifying, so you assume that the issue must be coming from your primers or PCR conditions. Briefly describe 2 different strategies for solving the problem and how they work.
Can you answer and explain it please Q. You want to PCR amplify the underlined/bold DNA...
Can you answer and explain it please
Q. You want to PCR amplify the underlined/bold DNA fragment. Design two primers for this job. The size of each primer is 18 bp -3 Forward primer: 5'- Reverse primer: 5'-( ATGACTATAGGGAACACTACGAGTGTGACAACGGTACGTAAC GCGGCGATCGAGCG ACCGAA GCTACTCCGCCTTTAGCTCTCGACAAT ATCGACGCCGCTA CGGATGTGTTTAGGCCTGGT GA TGCTGG AACCCTTTA TTCGCCGAGGAAG ATGTTATCAGCGGGTCTATCAGCATCA CGATCATCGTGAGCT GGTTCTTGGCTATTTCGGAGCAGGT GTCGAATCCGATGCT ITTGTGGGTGAGATGGGTTTATTCA GTGAAGTC ATCTTACGTACACGGA ACTCAGTGTGAATTGG AAGGC AGTTTAGCGAGTGATGCGCCGCGGATTTTGTATGCGATAGGTAT GCAGTTATCTAAGCGTTTATTGCTTACCAC
You are provided with two primers below and you wish to amplify the CYC1 terminatoron pMD1:...
You are provided with two primers below and you wish to amplify the CYC1 terminatoron pMD1: Primer 1: CTATGAAACAAGAATCGACC Primer 2: GTGCCGTAAAGCACTAAATC a. What secondary structure is formed by each primer at 54°C? b. What secondary structure is formed by both primers together at 54°C? c. If we were to use the following PCR protocol with primer 1 and primer 2, what would we expect to see on the electrophoresis gel? Step Time Temperature Initiation 2’ 95°C Denaturation 1’ 95°C...
Which of the following components is NOT used in the technique of PCR to amplify a specific DNA sequence in a mouse genome? O Specific single-stranded DNA primers O Double-stranded genomic mouse DNA O Heat-stable DNA polymerase D DNA ligase
Choose the two primers from the list that will amplify the region indicated by the bracket. The PCR product should be 25 base pairs long. (The DNA has dotted lines every 5 base pairs to help with counting.) Choose TWO correct primers 4 points possible 5' ...TTTTGAAGGCCACACTTTCCCTCTCAAAGGCCCGGAACCC... 3' 3' ...AAAACTTCCGGTGTGAAAGGGAGAGTTTCCGGGCCTTGGG... 5' region to be amplified If image does not load, click here to access. 5'GGAAA 3 5'CCGGG 3 5'GAGAG 3 5'CACAC 3' a 5'CCTTT 3 n 5'TTTTG 3 5'TTCCG 3...
• 4. How many copies of the primers do you add to the PCR? You add 3 pl. of a 5 M solution of each primer, If you had 100 copies of the template DNA would there be enough primers to support 30 cycles of PCR? Remember the amount of template doubles (in theory) with each cycle. After 1 cycle there will be 2xthe starting amount, after 2 cycles 4 x the starting amount.
1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
Can you answer and explain it please
Q. You want to PCR amplify the underlined/bold DNA fragment. Design two primers for this job. The size of each primer is 18 bp -3 Forward primer: 5'- Reverse primer: 5'-( ATGACTATAGGGAACACTACGAGTGTGACAACGGTACGTAAC GCGGCGATCGAGCG ACCGAA GCTACTCCGCCTTTAGCTCTCGACAAT ATCGACGCCGCTA CGGATGTGTTTAGGCCTGGT GA TGCTGG AACCCTTTA TTCGCCGAGGAAG ATGTTATCAGCGGGTCTATCAGCATCA CGATCATCGTGAGCT GGTTCTTGGCTATTTCGGAGCAGGT GTCGAATCCGATGCT ITTGTGGGTGAGATGGGTTTATTCA GTGAAGTC ATCTTACGTACACGGA ACTCAGTGTGAATTGG AAGGC AGTTTAGCGAGTGATGCGCCGCGGATTTTGTATGCGATAGGTAT GCAGTTATCTAAGCGTTTATTGCTTACCAC
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