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If you cut a circular plasmid with enzyme A that shows 2 enzyme A (A1 and...

If you cut a circular plasmid with enzyme A that shows 2 enzyme A (A1 and A2) marked with specific base pairs, is the 0 rest mark also considered as a band? If there is enzyme A1 and enzyme A2 on a plasmid, are there 2 bands or 3?

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Answer #1

If there is a circular plasmid containing two types of restriction sites for enzyme A1 and A2, and they are cut with these two enzymes, and assuming they have only one site each...then there will be 2 bands formed.

Now, if you will see the below picture, you will know how three bands are formed from a cirular band

0 o A2 Art A1

If your meaning of saying the 0 mark on plasmid is also a band, then yes..there will be two bands for it, one will be having 0 mark for it

Hope it helps, Good luck !!!!

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