What would be the most likely effect of a mutation in each of the following in...
What would be the most likely effect of a mutation in each of the following in an E. coli genome? (A) -8; (B) -35; (C) -20; (D) Start site.
Homework Answers
Answer:
(A) Mutation at the -8 position would probably affect the -10
consensus sequence (TATA box), which is centered on the position
-10. This sequence is necessary for the binding of RNA polymerase.
A mutation there would most likely cause a decrease in
transcription.
B) A mutation in the -35 region could affect the binding of sigma
factor to the promoter. Transcription would probably be reduced or
inhibited.
C) The -20 region is located between the consensus sequences of an
E.coli promoter. A mutation here may not have any effect
on transcription.
D) A mutation in the start site would have little effect on
transcription. The position of start site relative to the promoter
is more important than the sequence at the start site.
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