What enzyme activities and DNAs are required to clone a fragment of mouse DNA?
What enzyme activities and DNAs are required to clone a fragment of mouse DNA?
Homework Answers
The things that would be required for mouse DNA cloning
1. Plasmid DNA where the mouse DNA needs to be cloned
2. Primer DNA oligos which helps in amplifying the region of interest from mouse DNA
3. Suitable restriction endonucleases that makes uniques cuts in the plasmid and at two ends of the amplifies DNA.
4. TaqDNA polymerase for PCR amplification of target DNA
5. DNA ligase for ligating the traget DNA into plasmid DNA.
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What enzyme activities and DNAs are required to clone a fragment
of mouse DNA?
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Easy question
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A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
Easy question
Given that a DNA fragment law the end (on right): Which one of the following DNA ends is com, Place the following steps in chronological order when creating a tomato genomic DNA library. The available materials are DNA ligase, restriction enzyme, tomato leaves, plasmid DNK to act as vector that has already been digested with the restriction enzyme, competent bacteria, and nutrient agar plates containing ampicillin. You may use a step more than once. Add DNA ligase Transform...
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