Question

2. Which technique would you use to accomplish each of the following:

a. amplifying DNA __________________________________________________________

b. a vector to carry target DNA into bacteria _______________________________________

c. produce a double stranded cut in DNA ­­­­­­­­­­­­­­­­­­­­­­­­­­­_________________________________________

d. separating DNA fragments by size ____________________________________________

Answer 1

a. amplifying DNA :– PCR ( polymerase chain reaction)

PCR is a technique used to make numerous copies of a specific segment of DNA quickly and accurately.

b. a vector to carry target DNA into bacteria :–

Plasmid vectors are modified forms of the circular extra-chromosomal DNA molecules found in bacteria, which have been engineered to contain restriction sites and marker genes to allow the detection of bacterial cells that contain the plasmid.

c. produce a double stranded cut in DNA :–

Using Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences.

d. separating DNA fragments by size :–

Gel electrophoresis is a technique used to separate DNA fragments according to their size.