Question

Why can’t eukaryotic genes be used to express protein in bacteria?

a. They lack stop codons

b. They are too large

c. They use different nucleic acids in their DNA

d. They contain introns

e. They cannot be replicated

Answer 1

Answer:

Option D) they contain introns

Generally eukaryotic genome is complex in nature when compared to prokaryotes. One main difference between prokaryotes and eukaryotes is prokaryotes do not have introns their transcription and translation process is coupled,whereas eukaryotes have introns (non coding sequence) and their genome is larger in size therefore in protein synthesis process , to produce a functional protein RNA processing and post translational process is required.

Therefore utilising eukaryotic Gene in bacteria ,bacteria can not process the eukaryotic Gene with introns leads to synthesis of inactive proteins.

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Answer 2

The correct answer is d. They contain introns.

Eukaryotic genes contain introns, which are non-coding sequences within the gene that need to be removed during the process of gene expression. Bacteria lack the necessary machinery to accurately remove introns from eukaryotic genes. In contrast, bacteria have a simpler gene structure without introns, and their gene expression machinery is adapted to work with these non-intronic genes.

In addition to introns, eukaryotic genes may also have other regulatory sequences and elements that are specific to eukaryotic cells, which bacteria may not recognize or respond to appropriately. These differences in gene structure and regulation make it challenging for bacteria to correctly express proteins from eukaryotic genes.

Therefore, eukaryotic genes cannot be directly used to express proteins in bacteria without modifications or adaptations to overcome the presence of introns and other eukaryotic-specific elements.