10. The concentrations for each test are determined in the units; mg. protein per tube and...
10. The concentrations for each test are determined in the units; mg. protein per tube and mg. protein per ml. of sample. Are these values numerically the same? Explain. ______________________________________________________________________________ _____________________________________________________________________________
Homework Answers
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10. The concentrations for each test are determined in the
units; mg. protein per tube and...
1. Calculate concentrations for each test tubes in mg/mL. 20mL of stock Brilliant Blue solution is added to test tube #1...
1. Calculate concentrations for each test tubes in mg/mL. 20mL of stock Brilliant Blue solution is added to test tube #1. The concentration of this stock solution is 0.025g/L. a) Test tube #2, 10mL distilled water + 10 mL of stock Brilliant Blue solution from test tube #1. b) Test tube #3, 10mL of distilled water + 10mL of solution from test tube 2. c) Test tube #4, 10mL of distilled water + 10mL of solution from test tube 3....
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent ...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1...
Pre
lab quiz question
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the sample for a tube using 0.6 mL of the 2.0 mg/ml standard BSA Your Answer: Answer units
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
In order to determine the molar mass of an unknown protein, 368.7 mg of the protein...
In order to determine the molar mass of an unknown protein, 368.7 mg of the protein is dissolved in enough water to give 50.00 mL of solution. The osmotic pressure of this solution is determined to be 2.94 mmHg at 298 K. Estimate the molar mass of this protein. Give an answer to the 100's. Do not include units in your response, only digits.
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
26. The mRNA for a well-known secretory protein is isolated and placed in a test tube...
26. The mRNA for a well-known secretory protein is isolated and placed in a test tube in the presence of all the substances (or reagents) required for in vitro protein synthesis. The amino acid sequence of the protein produced in vitro (in a test tube) is then compared to the sequence of the purified secretory protein (purified from a eukaryotic cell). The sequences of the two proteins are not the same. Please give a possible explanation for this observation. Think...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Pre
lab quiz question
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the sample for a tube using 0.6 mL of the 2.0 mg/ml standard BSA Your Answer: Answer units
Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
In order to determine the molar mass of an unknown protein, 368.7 mg of the protein is dissolved in enough water to give 50.00 mL of solution. The osmotic pressure of this solution is determined to be 2.94 mmHg at 298 K. Estimate the molar mass of this protein. Give an answer to the 100's. Do not include units in your response, only digits.
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
26. The mRNA for a well-known secretory protein is isolated and placed in a test tube in the presence of all the substances (or reagents) required for in vitro protein synthesis. The amino acid sequence of the protein produced in vitro (in a test tube) is then compared to the sequence of the purified secretory protein (purified from a eukaryotic cell). The sequences of the two proteins are not the same. Please give a possible explanation for this observation. Think...
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