The plant gene of interest containing the promoter, control regions must be cloned and joined in the right orientation with glucuronidase gene. The glucuronidase gene locates the reporter gene under the same transcriptional control like the gene of interest.
In addition, transgenic plants are created by the transformed plant cells. The reporter gene is grown in a clump or callus, which can be produced to develop shoots and roots. The calli are moved to soil to develop into transgenic plants.
Furthermore, the glucuronidase gene will immediately express in the same developmental model like the gene of interest. The expression of the gene can simply be watched by dipping the plant in an X-Glu solution and analysing the blue reaction product.