Amplification of the mutant sequence by polymerase chain reaction (PCR) and sequencing it will help to verify the mutant one. In the haploid fungus Neurospora, the position of the gene for the protein actin is known from the complete genome sequence. If a slow-growing mutant that was suspected of being actin mutant, then amplify the mutant sequence by using PCR and then sequence the gene.
In most recent years, the search for mutations and sequence polymorphisms has been significantly accelerated by the use of PCR and then by direct sequencing of PCR products. The enormous advantage of PCR is that less process is needed when related with cloning.
The slow-growing mutant is most easily sequenced by using PCR without actually cloning the DNA. Though it is required that the sequence be identified, the primers are present previously in the genome and are adequately close. When the situations meet up, the primers find out the specificity of the DNA segment amplified and therefore would be most effective.