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Solutions For An Introduction to Genetic Analysis Chapter 10 Problem 27P

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Solution 1

631190-10-27P AID: 2498 | 25/08/2015

a.

PCR (polymerase chain reaction) is suitable for isolating the same gene from 20 individuals. PCR uses specific primers to produce multiple copies of DNA (deoxyribonucleic acid) from a very little amount of sample. Isolating the DNA samples from 20 individuals and exposing them to PCR reaction using specific primers would give target DNA.

b.

Gene cloning is preferable for isolating 100 genes from the same individual. A gene clone contains variety of genes. They can be identified by target specific probes.

c.

Gene cloning is preferable to achieve this goal. Because a rat genome clone contains number of genes isolated from a rat. Exposing the rat clones to mouse specific DNA probe may hybridize with rat genes, and the resulting hybrid can be identified as mouse gene.

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