Problem

Solutions For An Introduction to Genetic Analysis Chapter 10 Problem 32P

Step-by-Step Solution

Solution 1

Sanger’s DNA (deoxyribonucleic acid) sequencing method is also called chain-termination DNA sequencing or dideoxy DNA sequencing. The sequencing involves use of template DNA mixed with oligonucleotide primers, DNA polymerase, the four deoxy nucleoside triphosphates (dNTPs), and the four dideoxynuleoside triphosphates (ddNTPs). The synthesis of new strands upon the primers occurs until a ddNTP is added to the growing polymer. The ddNTPs lack -OH groups which are required for the -PO4 of the incoming nucleotides to form phosphodiester bonds. As a result, polymerization of the chain terminates.

Step #2 of 5

A cloned frament of DNA (deoxyribonucleic acid) is sequenced by using dideoxy chain-tremination method.

The autoradioram of the sequencing gel is represented below:

Step #3 of 5

The gel can be read from the bottom to the top in a 5' to 3' direction. The nucleotide sequence of the DNA nucleotide chain produced from the primer is as follows: