You have measured the following data on the destruction of thiamine by an enzyme, both with and without 0.2 X 10-4 M inhibitor (o-aminobenzyl-4-methylthiazolium chloride).
[Thiamine], mM | Velocity, M min1 | |
| Uninhibited | Inhibited |
0.10 | 0.746 X 10-6 | 0.136 X 10-6 |
0.25 | 0.992 X 10-6 | 0.285 X 10-6 |
0.50 | 1.12 X 10-6 | 0.618 X10-6 |
1.00 | 1.26 X 10-6 | 0.758 X 10-6 |
2.00 | 1.36 X 10-6 | 1.29 X 10-6 |
a. Make suitable plots and use the Vmax and KM values to determine the type of inhibition.
b. Based on these results, determine the binding constant for the inhibitor.
We need at least 10 more requests to produce the solution.
0 / 10 have requested this problem solution
The more requests, the faster the answer.