The decarboxylation of a β -keto acid catalyzed by a decarboxylation enzyme can be measured by the rate of CO2 formation. From the initial rates given in the table, usinga method of your choice, determine the Michaelis-Menten constant for the enzyme and the maximum velocity by a graphical method.
Keto acid concentration [M] | Initial velocity [μmol CO2/(2 min)] |
2.500 | 0.588 |
1.000 | 0.500 |
0.714 | 0.417 |
0.526 | 0.370 |
0.250 | 0.256 |
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