Sodium succinate S is oxidized in the presence of dissolved oxygen to form sodium fumarate F in the presence of the enzyme succinoxidase E. The following table gives the i nitial velocity V0 of this reaction at several succinate concentrations:
[S] (mM) | 10 | 2.0 | 1.0 | 0.50 | 0.33 |
1 v0,μMs-1 | 1.17 | 0.99 | 0.79 | 0.62 | 0.50 |
a. Make a suitable plot of the data to test whether they follow the Michaelis-Menten mechanism. Draw the best line or curve through the data.
b. Calculate values for the maximum velocity Vmax and the Michaelis constant KM for this reaction. Show how you obtain them from the plot.
c. Malonate is a competitive inhibitor of this reaction. In an experiment with initially 1.0 mM succinate, the initial velocity is decreased by 50% in the presence of 30 mM malonate. Use this result to construct a second curve or line on the plot showing the expected kinetic behavior of this reaction in the presence of 30 mM malonate.
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