Consider the enzyme-catalyzed hydrolysis of an ester that is competitively inhibited by Mg2+. The following data were obtained: KM = 10-3 M, Ki = 10-4 M and V = 1 M s-1. M I max
a. Estimate how long it will take for 10-5 M substrate to become 10-6 M in the absence of inhibitor.
b. Estimate how long it will take 1 M substrate to become 10-1 M in the absence of inhibitor.
c. Repeat calculations for parts (a) and (b) in the presence of 10-4 M Mg2+.
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