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If you forget to heat fix the specimen in simple staining, how will it impact the appearance of the specimen
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(Q). If you forget to heat fix the specimen in simple staining, how will it impact the appearance of the specimen?

Ans : Before simple or any type of staining, smear of specimen must be made on a slide and heat fixation occured. During heat fixing, it helps to adherence of specimen's cells to the slide.
We mainly use simple staining for bacterial staining. Heat fix denatures bacterial enzymes that prevent them from digesting cell parts.
During the staining, we wash the side (that contains bacteria) several times with water and 70% alcohol or acetone and kept the slide for air dry. If we do not heat fix the specimen, we will wash off the specimen. So, we will not able to see any type of specimen or a little amount of specimen under the microscope.

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