(Q). If you forget to heat fix the specimen in simple staining, how will it impact the appearance of the specimen?
Ans : Before simple or any type of staining, smear of specimen
must be made on a slide and heat fixation occured. During heat
fixing, it helps to adherence of specimen's cells to the
slide.
We mainly use simple staining for bacterial staining. Heat fix
denatures bacterial enzymes that prevent them from digesting cell
parts.
During the staining, we wash the side (that contains bacteria)
several times with water and 70% alcohol or acetone and kept the
slide for air dry. If we do not heat fix the specimen, we will wash
off the specimen. So, we will not able to see any type of specimen
or a little amount of specimen under the microscope.
If you forget to heat fix the specimen in simple staining, how will it impact the...
What is the purpose of the heat-fix step in the direct staining technique? A heat allows the cells to absorb the stain O B cells attach to the slide с cells separate creating a monolayer D this step kills bacteria
You need to prepare a paraformaldehyde (PFA) solution in order to fix cells for staining. You begin with a 32% paraformaldehyde solution. The protocol asks for a 4% paraformaldehyde fixation. If you want to make 15mL of 4% paraformaldehyde how much 32% paraformaldehyde needs to be added to what volume of PBS
what will happen to a Gram positive cell that you’re Gram staining(usually purple? if you forgot to heat fix it?
1. There are other staining procedures beyond simple staining and differential staining. Examples include endospore, acid-fast, capsule, and flagella stains. Please pick one staining technique to research and answer the following questions: • What is the theory behind the staining technique, what stains are used, how are they applied, what portion of the bacteria are they reacting with • How is this stain used to differentiate or identify bacterial cells or cell structures? • Describe the staining technique. List and...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
1. Who can specimen labeling error impact nursing practice setting. And how can it impact by research evidence 2. Why is this issue important? Why does it matter? Wholwhat is negatively impacted?
1: Who can specimen labeling error impact nursing practice setting. And how can it impact by research evidence 2: Why is this issue important? Why does it matter? Who/what is negatively impacted?
-). You have a specimen in focus on the 40X objective magnification. Detail the steps needed to view the specimen at 100X objective magnification. (don't forget to mention the oil!)