why are plasmids easier to study than genomic DNA?
Homework Answers
Genomic DNA provides all the information for regular well-being of the cell, while plasmid DNA provides extra characteristics to a cell, like antibiotic resistance, which can allow a cell to survive in harsh conditions. Genomic DNA is very large in comparison to plasmid DNA hence it is easier to study the plasmid DNA than to study whole genomic DNA. Also, plasmids are distinct from chromosomes in being non-essential.
Why is an intermediate like mRNA needed to copy the information from the genomic DNA so...
Why is an intermediate like mRNA needed to copy the information from the genomic DNA so it can be translated into proteins? Use an example to explain why would you need to extract genomic DNA? What is a plasmid? Where are plasmids found? Explain HOW plasmids play a role in the development of multiple drug resistant strains of bacteria
How to interpret the gel results following plasmid isolation (i.e., differentiate between plasmids, genomic DNA, and...
How to interpret the gel results following plasmid isolation (i.e., differentiate between plasmids, genomic DNA, and RNA)? ** I have a lab exam on this subject, could someone give me an example?
Why is alkaline SDS used for plasmid DNA isolation but not for genomic DNA isolation?
Why is alkaline SDS used for plasmid DNA isolation but not for genomic DNA isolation?
8. Your lab assistant has tried to construct a genomic library of recombinant plasmids using only...
8. Your lab assistant has tried to construct a genomic library of recombinant plasmids using only the EcoRI restriction enzyme, genomic DNA, a plasmid and DNA ligase. The plasmid used is a typical cloning plasmid with a multiple cloning site (MCS) in the middle of a Lacz gene. After transforming E. coli with the library, a few white colonies are observed but a most of the colonies are blue. Explain what happened and what you would tell your assistant to...
What is cloning? Why is it necessary to clone genomic DNA fragments when sequencing a genome...
What is cloning? Why is it necessary to clone genomic DNA fragments when sequencing a genome by the Sanger Shotgun approach?
In order for a human genomic DNA library to fully represent the entire genomic information content...
In order for a human genomic DNA library to fully represent the entire genomic information content of humans, what type of methods must be employed to fractionate the human genomic DNA for making the library?
Why do some plasmids have higher copy numbers than others?
Why do some plasmids have higher copy numbers than others?
And.. Exercise1: Give the basic steps involved in extracting genomic DNA from animal cells and tossues?...
And..
Exercise1:
Give the basic steps involved in extracting genomic
DNA from animal cells and tossues?
23- Which of the following statements is correct? a. Longer DNA fragments migrate farther than shorter fragments. b. Migration distance is inversely proportional to the fragment size. c. Positively charged DNA migrates more rapidly than negatively charged DNA. d. Uncut DNA migrates farther than DNA cut with restriction enzymes. 24- Why do scientists load DNA of known sizes (also called "marker" or "ladder") into...
Human genomic DNA is around 3 billion bps. Why do we see a band around 10kb...
Human genomic DNA is around 3 billion bps. Why do we see a band around 10kb in 1% agarose gel electrophoresis? Did we do something wrong, damage the DNA and shorten it or something, what kb shall we expect to see it?
genomic DNA extraction proticol queations 1- why is sodium Azide added in wash buffer 2 2-what...
genomic DNA extraction proticol queations 1- why is sodium Azide added in wash buffer 2 2-what is the purpose of elution buffer (AE)? 3- why the filter spined even after there is no liquid remaining?
Why is an intermediate like mRNA needed to copy the information from the genomic DNA so it can be translated into proteins? Use an example to explain why would you need to extract genomic DNA? What is a plasmid? Where are plasmids found? Explain HOW plasmids play a role in the development of multiple drug resistant strains of bacteria
8. Your lab assistant has tried to construct a genomic library of recombinant plasmids using only the EcoRI restriction enzyme, genomic DNA, a plasmid and DNA ligase. The plasmid used is a typical cloning plasmid with a multiple cloning site (MCS) in the middle of a Lacz gene. After transforming E. coli with the library, a few white colonies are observed but a most of the colonies are blue. Explain what happened and what you would tell your assistant to...
What is cloning? Why is it necessary to clone genomic DNA fragments when sequencing a genome by the Sanger Shotgun approach?
And..
Exercise1:
Give the basic steps involved in extracting genomic
DNA from animal cells and tossues?
23- Which of the following statements is correct? a. Longer DNA fragments migrate farther than shorter fragments. b. Migration distance is inversely proportional to the fragment size. c. Positively charged DNA migrates more rapidly than negatively charged DNA. d. Uncut DNA migrates farther than DNA cut with restriction enzymes. 24- Why do scientists load DNA of known sizes (also called "marker" or "ladder") into...
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