How would you make a 50mL of a 1X solution starting with a 10X solution and water?
We should use the formula
M1V1 = M2V2.
1X x 50 mL =10X x ( X ml)
X = 5 ml.
So, you should mix 45 ml of water and 5 ml of 10X solution.
How would you make a 50mL of a 1X solution starting with a 10X solution and...
You currently have 20,000X ethidium bromide. You want to make 250 mL of 1X ethidium bromide dissolved in 1X TBE. You currently also have a stock of 10X TBE solution. How much 10X TBE do you need and how much 20,000X ethidium bromide do you need?
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If I want to make 50mL of 5M of NaCl, would I use: 14.6 grams of NaCl and 50 mL of water? Most of the examples I see online are for 1L of NaCl, not 50mL so i'm not sure. If that's not right, then please tell me how to make 50mL of 5M NaCl.
a) Calculate how you would make 1.0 L of 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW=121.4 g/mole; Boric Acid MW=61.84 g/mole; EDTA MW=292.2 g/mole b) For your polyacrylamide gel electrophoresis gel you need 50 mL of 0.5x TBE. Calculate and describe how you would make this solution using your stock solution.
how do you make 500ml of 1X SDS running buffer from a 5X stock solution?
You need to make a 50X stock solution for later use. The 1X solution requires 0.9 g/100 mls final. So, to make 500 mls of 50X stock solution, how many grams will you add?
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