If x gene was an auxotroph for Histidne, and potencially escencial, how would you prove this...
- If x gene was an auxotroph for Histidne, and potencially escencial, how would you prove this proposition?
Homework Answers
To determine whether a given gene is essential for an organism, we have to observe its phenotype under selective conditions.
In the given case, it is stated that
gene X is an auxotroph for Histidine.
To determine if gene X is essential, plate the mutant cells (Loss
of function for gene X) on a media without Histidine.
If the mutant cells are able to grow, it shows that gene X is not
essential.
If the mutant cells fail to grow, it shows that gene X is
essential.
Add Answer to:
If x gene was an auxotroph for Histidne, and potencially
escencial, how would you prove this...
What is an auxotroph? How were auxotroph's used to demonstrate one-gene coded for one enzyme. Explain...
What is an auxotroph? How were auxotroph's used to demonstrate one-gene coded for one enzyme. Explain how RNA and DNA migrate in a sucrose gradient when mixed compared to when they are mixed, heated and allowed to slow cool. What gives you a difference in the behavior of the RNA peak?
Sceintists can identify the gene mutated in a bacterial auxotroph by transforming the cells with a...
Sceintists can identify the gene mutated in a bacterial auxotroph by transforming the cells with a genomic library in whihc fragments of the auxotroph's genome have been cloned into plasmids. Select one: a. False b. True
In the lab, you have a strain that is normally an auxotroph. You conduct a mutagenesis...
In the lab, you have a strain that is normally an auxotroph. You conduct a mutagenesis on this strain to try and 'rescue' that phenotype (make it no longer an auxotroph). How would you go about selecting for mutant strains that are no longer auxotrophs? a) Add in a compound (like an antibiotic) to kill the bacteria that did not mutate. b) Remove the key nutrient from the growth medium. c) By using transduction.
How can you prove that your chosen candidate is the CFTR gene? Select the three correct...
How
can you prove that your chosen candidate is the CFTR gene? Select
the three correct answers.
Problem 16.15 Mutations in the CFTR gene result in cystic fibrosis in humans, a condition in which abnormal secretions are present in the lungs, pancreas, and sweat glands. In the effort to positionally clone the CFTR gene, the gene was mapped to a region of 500 kb on chromosome 7 containing three candidate genes. Part A Using your knowledge of the disease symptoms,...
In order to prove this proposition: (P (x)-+ Q (z)) <-(R (z) Л Q (z))you must prove which of the following propositi...
In order to prove this proposition: (P (x)-+ Q (z)) <-(R (z) Л Q (z))you must prove which of the following propositions? Select all (if any) that apply. B) (Q (z) л P(x)) (R (z) Q (z)) F) All of the Above G) None of The Above
In order to prove this proposition: (P (x)-+ Q (z))
Biotechniques You are interested in studying the effects of glucose on Gene X. Your hypothesis is...
Biotechniques
You are interested in studying the effects of glucose on Gene X. Your hypothesis is that expression of Gene X will increase/decrease under high glucose conditions. To prove this, design 5 experiments using the following techniques: Reporter assay sensitivity assay EMSA blot In situ hybridization (IHC with probe for RNA) For each experiment, answer the following questions: What does the technique tell you (what does it measure)? How does this information help support your hypothesis (what part of the...
what is the function of the akh gene? And how would you purify the AKH protein...
what is the function of the akh gene? And how would you purify the AKH protein to verify the expression of the akh gene?
You hypothesize that expression of Gene X, a gene for which you know the complete sequence...
You hypothesize that expression of Gene X, a gene for which you know the complete sequence in the organism of interest, may be an important contributor to the development of a phenotype (trait) that you study (e.g. eye development). What approach(es) would you use to manipulate the expression pattern of the gene to test your hypothesis?
Describe how you would study/characterize an unknown eukaryotic gene promoter (what methods you would use, what...
Describe how you would study/characterize an unknown eukaryotic gene promoter (what methods you would use, what you would be looking for).
Prove Proposition 10.10 i Proposition 10.10. Let x,y,zER. (i) |x-y| = 0 ifand only ifx =...
Prove Proposition 10.10 i
Proposition 10.10. Let x,y,zER. (i) |x-y| = 0 ifand only ifx = y.
What is an auxotroph? How were auxotroph's used to demonstrate one-gene coded for one enzyme. Explain how RNA and DNA migrate in a sucrose gradient when mixed compared to when they are mixed, heated and allowed to slow cool. What gives you a difference in the behavior of the RNA peak?
How
can you prove that your chosen candidate is the CFTR gene? Select
the three correct answers.
Problem 16.15 Mutations in the CFTR gene result in cystic fibrosis in humans, a condition in which abnormal secretions are present in the lungs, pancreas, and sweat glands. In the effort to positionally clone the CFTR gene, the gene was mapped to a region of 500 kb on chromosome 7 containing three candidate genes. Part A Using your knowledge of the disease symptoms,...
In order to prove this proposition: (P (x)-+ Q (z)) <-(R (z) Л Q (z))you must prove which of the following propositions? Select all (if any) that apply. B) (Q (z) л P(x)) (R (z) Q (z)) F) All of the Above G) None of The Above
In order to prove this proposition: (P (x)-+ Q (z))
Biotechniques
You are interested in studying the effects of glucose on Gene X. Your hypothesis is that expression of Gene X will increase/decrease under high glucose conditions. To prove this, design 5 experiments using the following techniques: Reporter assay sensitivity assay EMSA blot In situ hybridization (IHC with probe for RNA) For each experiment, answer the following questions: What does the technique tell you (what does it measure)? How does this information help support your hypothesis (what part of the...
Describe how you would study/characterize an unknown eukaryotic gene promoter (what methods you would use, what you would be looking for).
Prove Proposition 10.10 i
Proposition 10.10. Let x,y,zER. (i) |x-y| = 0 ifand only ifx = y.
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