The addition of a “5ʹ-cap” and a “3ʹ poly-A” tail to many nuclear RNA species occurs prior to export of mature mRNAs to the cytoplasm. Such modifications appear to influence mRNA stability. Many assays for gene regulation involve the use of reporter genes such as luciferase from the North American firefly (Photinus pyralis). When the luciferase gene is transcribed and translated, the protein product can be easily assayed in a test tube. In the presence of luciferin, ATP, and oxygen, the luciferase enzyme produces light that can be easily quantified. Assuming that luciferase mRNA can be obtained and differentially modified (5ʹ-capped, poly-A tailed) in a test tube, suggest an assay system that would allow you to determine the influence of 5ʹ-capping and poly-A tail addition on mRNA stability.
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