In a classical genetic analysis, scientists create mutations within or surrounding cloned gene sequences. They then introduce these mutated gene sequences into cell cultures or model organisms and examine the phenotypes. Geneticists are now adding RNA-induced gene silencing to their gene-analysis toolbox. Describe how you would use RNA-induced gene silencing to study the role of the Drosophila tra gene in sex determination. Next, describe how you would study tra function, using a classical genetic analysis. What are the advantages and limitations of each research approach?
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