In this chapter, we focused on how eukaryotic genes are regulated at different steps in their expression, from chromatin modifications to control of protein stability. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter,
(a) How do we know that promoter and enhancer sequences control the initiation of transcription in eukaryotes?
(b) How do we know that eukaryotic transcription factors bind to DNA sequences at or near promoter regions?
(c) How do we know that double-stranded RNA molecules can control gene expression?
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