Problem

Explain how the following mutations would affect the transcription of the yeast GAL1 gene....

Explain how the following mutations would affect the transcription of the yeast GAL1 gene.

(a) A deletion within the GAL4 gene that removes the region encoding amino acids 1 to 100.


(b) A deletion of the entire GAL3 gene.


(c) A mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p.


(d) A deletion of one of the four UASG elements upstream from the GAL1 gene.


(e) A point mutation in the GAL1 core promoter that alters the sequence of the TATA box.

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