Solutions For An Introduction to Genetic Analysis Chapter 5 Problem 6P

The final diagram represents that gal gene is entering the donor at 25 minutes.

In the above interrupted-mating conjugation experiment, F^- streptomycin-resistant cells with mutations in azi, ton, lac, and gal are incubated for varying times with Hfr cells that are sensitive to streptomycin and carry wild-type alleles for these genes.

Below is the schematic view of the transfer of markers:

The gal gene has the maximum transfer frequency of approximately 25%.

The azi, ton, and lac genes have all entered at 25 min. They all can become part of a stable trans-conjugant.